Abstract
The cross-reactivity of several monoclonal antibodies recognizing monomorphic determinants of human HLA-A, B, C, and DR antigens and human β2-microglobulin (β2m) has been studied on peripheral blood leukocytes in 24 different species. An monoclonal HLA-A-, B-, and C-specific antibody and four monoclonal HLA-DR-specific antibodies cross-reacted with cells from all the primate species tested. Furthermore, antibodies HLA-DR-specific were positive with peripheral blood leukocytes (PBL) from cows, goats, sheep, horses, and dogs. Two monoclonal β2m-specific antibodies, which were positive with PBL from certain primates, also reacted with cells from cows, goats, sheep, horses, and dogs. Two other #2m-specific antibodies reacted only with PBL from chimpanzees. No reaction could be detected with all our reagents in other classes tested (birds, reptiles, amphibians, and Teleostei).
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Armerding, D., Sachs, D. H., and Katz, D. H.: Activation of T and B lymphocytes in vitro. III. Presence of la determinants on allogeneic effect factor. J. Exp. Med. 140: 1717–1722, 1974
Balner, H., van Leeuwen, A., Van Vreeswiik, W., Dersjant, H., and van Rood, J. J.: Leukocyte antigens of rhesus monkeys (Rh L-A) and chimpanzee (Ch L-A): similarities with the human HL-A system. Tissue Antigens 1: 229–238, 1971
Brodsky, F. M., Bodmer, W. F., and Parham, P.: Characterization of a monoclonal anti-β2-microglobulin antibody and its use in the genetic and biochemical analysis of major histocompatibility antigens. Eur. J. Immunol. 9: 536–545, 1979
Colombani, J., Colombani, M., Degos, L., David, C. J., and Shreffler, D. C.: Reactivity of anti-HLA sera against mouse lymphocytes. Tissue Antigens 9: 111–120, 1977
Dorf, M. E., Toth, E. K., and Balner, H.: Cross-reaction of HL-A antibodies: V-Relationship between the human HL-A11 and chimpanzee ChW-11 specificities. Tissue Antigens 2: 461–472, 1972
Fridman, W. H., Rabourdin-Combe, C., Neauport-Sautes, C., and Gisler, R. H.: Characterization and function of T-cell Fcγ receptor. Immunol. Rev. 56: 51–88, 1981
Gates, F. T., Coligan, J. E., and Kindt, T. J.: Complete amino-acid sequences of rabbit β2-microglobulin. Biochemistry 18: 2267–2272, 1979
Gordon, S. M. and Kindt, T. J.: Detection of β2-microglobulin in sera from diverse vertebrate species by the use of a heterologous radioimmunoassay. Scand. J. Immunol. 5: 505–511, 1976
Kapp, J. A.: Immunosuppressive factor(s) extracted from lymphoid cells of non-responder mice primed with L-glutamic acid 60-L-alanine-L-tyrosine10 (GAT). IV. Lack of strain restriction among allogeneic non-responder donors and recipients. J. Exp. Med. 147: 997–1006, 1978
Lipinsky, M. Fridman, W. H., Tursz, T., Vincent, C., Pious, D., and Fellous, M.: Absence of allogeneic restriction in human T cell mediated cytotoxicity to Epstein-Barr virus-infected target cells. Demonstration of an HLA-linked control at the effector level. J. Exp. Med. 150: 1310–1322, 1979
McMichael, A. J., Parham, P., Brodsky, F. M., and Pilch, J. R.: Influenza virus-specific cytotoxic T lymphocytes recognize HLA-molecules. Blocking by monoclonal anti-HLA antibodies. J. Exp. Med. 152: 195s-203s, 1980
Michaelson, J., Rothenberg, E., and Boyse, E. A.: Genetic polymorphism of murine β2-microglobulin detected biochemically. Immunogenetics 11: 93–95, 1980
Parham, P., Sehgal, P. K., and Brodsky, F. M.: Anti-HLA-A, B, C monoclonal antibodies with no alloamigenic specificity in human define polymorphisms in other primate species. Nature 279: 63–641, 1979
Rabourdin-Combe, C., Dorf, M. E., Guimezanes, A., and Fridman, W. H.: T-cell produced immunoglobulin binding factor (IBF) bears determinants coded for by the I region of the major histocompatibility complex and lacks allogeneic restriction. Ear. J. Immunol. 9: 237–242, 1979
Waltenbaugh, C., Debré, P., Theze, J., and Benacerraf, B.: Immuno-suppressive factor(s) specific for L-glutamic acid 50-L-tyrosine50 (GT). I. Production, characterization, and lack of H-2 restriction for activity in recipient strains. J. Immunol. 118: 2073–2077, 1977
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Teillaud, JL., Crevat, D., Chardon, P. et al. Monoclonal antibodies as a tool for phylogenetic studies of major histocompatibility antigens and β 2-microglobulin. Immunogenetics 15, 377–384 (1982). https://doi.org/10.1007/BF00364261
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DOI: https://doi.org/10.1007/BF00364261