Abstract
Sequential immunoprecipitation and isoelectric focusing analyses with monoclonal I-E-specific antibodies presented in this paper indicate the existence of multiple I-E molecules. In sequential immunoprecipitations with 13-4 (anti-Ia.7) and 17-3-3 (anti-Ia.22) monoclonal antibodies, 17-3-3 only partially cleared I-E molecules immunoprecipitated by 13-4. Similarly, 13-4 monoclonal antibody only partially cleared I-E molecules precipitated by 17-3-3 monoclonal antibody. These results suggested a minimum of three I-E molecules. One I-E molecule expresses both I3-4 and I7-3-3 determinants, a second I-E molecule expresses only 17-3-3 determinants, and a third I-E molecule expresses only 13-4 determinants. Isoelectric focusing analyses of I-E molcules immuno-precipitated by 13-4 and 17-3-3 showed differences in both Ae beta polypeptide chains and Eα alpha polypeptide chains. The sequential immunoprecipitation and isoelectric focusing analyses presented in this paper can be explained by a model in which there are at least two separate Ae genes being encoded within the I-A subregion and two separate Eα genes being encoded within the I-E subregion. The 17-3-3 monoclonal antibody would recognize a determinant on only one of two Ae beta polypeptide chains and the 13-4 monoclonal antibody would recognize a determinant on only one of two Eα polypeptide chains.
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Abbreviations
- TAR:
-
torpedo acetylcholine receptor
- MLR:
-
mixed lymphocyte reaction
- GL-Phe:
-
poly(Glu55Lys36Phe9)
- LPS:
-
lipopolysaccharide
- SDS:
-
sodium dodecylsulfate
- IEF:
-
isoelectric focusing
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Lafuse, W.P., Corser, P.S. & David, C.S. Biochemical evidence for multiple I-E Ia molecules. Immunogenetics 15, 365–375 (1982). https://doi.org/10.1007/BF00364260
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DOI: https://doi.org/10.1007/BF00364260