Abstract
The Lyt-2 aallele of the C.AKR strain of mice (genotype Lyt-2 a, Lyt-3 a) was cloned, and its complete nucleotide sequence as well as that of 2 kb of 5′ flanking DNA was determined. The sequence was comapred with the partial sequence of the Lyt-2 aallele of DBA/2 (genotype Lyt-2 a, Lyt-3 b) and the nearly complete sequence of the B10.CAS2 Lyt-2 ballele reported by Liaw and coworkers (1986). The coding regions of the two Lyt-2 aalleles differ from each other by two nucleotide substitutions in the three exons over which they could be compared, resulting in two amino acid substitutions in the leader and transmembrane segments. The coding region of the C.AKR Lyt-2 aallele differs from the Lyt-2 ballele by two nucleotide substitutions in the extracellular V-like domain, one of which is silent and the second of which leads to substitution of valine for methionine at amino acid position 78 giving rise to the Lyt-2.1 allotypic specificity. The coding region of the DBA/2 Lyt-2 aallele shares with C.AKR the allotypic substitution at position 78 and differs from Lyt-2 bby three additional nucleotide substitutions in the coding regions, two of which lead to amino acid substitutions in the leader and transmembrane segments. It would therefore appear that the Lyt-2 alleles of the three strains analyzed are distinct, and the nomenclature Lyt-2 a1 and Lyt-2 a2 is suggested to distinguish the alleles of C.AKR and DBA/2, respectively. These alleles share a common difference from the Lyt-2 bgene product at position 78, and since the amino acid substitutions which distinguish them from each other are in the leader and transmembrane segments, their mature Lyt-2 gene products appear antigenically identical.
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References
Boyse, E. A., Miyazawa, M., Aoki, T., and Old, L. J.: Ly-A and Ly-B: two systems of lymphocyte isoantigens in the mouse. Proc R Soc London 170: 175–193, 1968
Boyse, E. A., Itakura, K., Stockert, E., Iritani, C. A., and Miura, M.: LyC: a third locus specifying alloantigens exposed only on thymocytes and lymphocytes. Transplantation 11: 351–352, 1971
Dembič, Z., Haas, W., Zamoyska, R., Parnes, J., Steinmetz, M., and Boehmer, H.: Transfection of the CD8 gene enhances T-cell recognition. Nature 326: 510–511, 1987
Durda, P. J. and Gottlieb, P. D.: The Ly-3 antigens on mouse thymocytes: immune precipitation and molecular weight characterization. J Exp Med 144: 476–493, 1976
Durda, P. J. and Gottlieb, P. D.: Sequential precipitation of mouse thymocyte extracts with anti-Lyt-2 and anti-Lyt-3 sera. I. Lyt-2.1 and Lyt-3.1 antigenic determinants reside on separable molecular species. J Immunol 121: 983–989, 1978
Frischauf, A.-M., Lehrach, H., Poustka, A., and Murray, N.: Lambda replacement vectors carrying polylinker sequences. J Mol Biol 170: 827–842, 1983
Gabert, J., Langlet, C., Zamoyska, R., Parries, J. R., Schmitt-Verhulst, A.-M., and Malissen, B.: Reconstitution of the MHC class I specificity by transfer of the T cell receptor and Lyt-2 genes. Cell 50: 545–554, 1987
Gillies, S. D., Morrison, S. L., Oi, V. T., and Tonegawa, S.: A tissuespecific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene. Cell 33: 717–728, 1983
Gottlieb, P. D.: Genetic correlation of a mouse light chain variable region markers with a thymocyte surface antigen. J Exp Med 140: 1432–1437, 1974
Gottlieb, P. D., Marshak-Rothstein, A., Auditore-Hargreaves, K., Berkoben, D. B., August, D. A., Rosche, R. M., and Benedetto, J. D.: Construction and properties of new Lyt-congenic strains and antiLyt-2.2 and anti-Lyt-3.1 monoclonal antibodies. Immunogenetics 10: 545–555, 1980
Gottlieb, P. D., Boyd, R. T., Ponath, P. D., and Goldrick, M. M.: Restriction enzyme polymorphisms in Vκ and Jκ genes of inbred and wild mice. Curr Top Microbiol Immunol 127: 186–192, 1986
Graham, F. L., Bacchetti, S., McKinnon, R., Stanners, C., Cordell, B., and Goodman, H. M.: Transformation of mammaliam cells with DNA using the calcium technique. In R. Baserga, C. Croce, and G. Rovera (eds.): Introduction of Macromolecules into Viable Mammaliam Cells, pp. 3–25, Alan R. Liss, Inc., New York, 1980
Itakura, K., Hutton, J. J., Boyse, E. A., and Old, L. J.: Genetic linkage relationships of loci specifying differentiation alloantigens in the mouse. Transplantation 13: 239–243, 1972
Kadonaga, J. T., Jones, K. A., and Tijan, R.: Promoter-specific activation of RNA polymerase II transcription by Spl. Trends Biochem 11: 20–23, 1986
Kavathas, P. and Herzenberg, L. A.: Transfection for lymphocyte cell surface antigens. In D. M. Weir (ed.): Handbook of Experimental Immunology, Fourth Edition, Volume 3, Blackwell Publications, Oxford, pp. 91.1–91.10, 1986
Kavathas, P., Sukhatme, V. P., Herzenberg, L. A., and Parties, J. R.: Isolation of a gene encoding the human T-lymphocyte differentiation antigen Leu-2 (T8) by gene transfer and cDNA subtraction. Proc Natl Acad Sci USA 81: 7688–7692, 1984
Ledbetter, J. A. and Herzenberg, L. A.: Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev 47: 63–90, 1979
Ledbetter, J. A., Seaman, W. E., Tsu, T. T., and Herzenberg, L. A.: Lyt-2 and Lyt-3 antigens are on two different polypeptide subunits linked by disulfide bonds. Relationship of subunits to T cell cytolytic activity. J Exp Med 153: 1503–1516, 1981
Liaw, C. W., Zamoyska, R., and Parties, P. R.: Structure, sequence, and polymorphism of the Lyt-2 T cell differentiation gene. J Immunol 137: 1037–1043, 1986
MacDonald, H. R., Glasebrook, A. L., Bron, C., Kelso, A., and Cerottini, J.-C.: Clonal heterogeneity in the functional requirement for Lyt-2/3 molecules on cytolytic T lymphocytes (CTL): possible implications for the affinity of CTL antigen receptors. Immunol Rev 68: 88–115, 1982
Maniatis, T., Fritsch, E. F., and Sambrook, J.: Molecular Cloning: A Laboratory Manual, pp. 1–545, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1983
Maxam, A. M. and Gilbert, W.: Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol 65: 499–560, 1980
Messing, J.: M13mp2 and derivatives: a molecular cloning system for DNA sequencing, strand-specific hybridization, and in vitro mutagenesis. In A. Walton (ed.): Third Cleveland Symposium on Macromolecules: Recombinant DNA, pp. 143–153, Elsevier North Holland, Amsterdam, 1981
Nakauchi, H., Nolan, G. P., Hsu, C., Huang, H. S., Kavathas, P., and Herzenberg, L. A.: Molecular cloning of Lyt-2, a membrane glycoprotein marking a subset of mouse lymphocytes: molecular homology to its human counterpart, Leu-2/T8, and to immunoglobulin variable regions. Proc Natl Acad Sci USA 82: 5126–5130, 1985
Poncz, M., Solowiejczyk, D., Ballantine, M., Schwartz, E., and Surrey, S.: “No-random” DNA sequence analysis in bacteriophage M13 by the dideoxy chain-termination method. Proc Natl Acad Sci USA 79: 4298–4302, 1982
Reilly, E. B., Auditore-Hargreaves, K., Hämmerling, U., and Gottlieb, P. D.: Lyt-2 and Lyt-3 alloantigens: precipitation with monoclonal and conventional antibodies and analysis on one- and two-dimensional polyacrylamide gels. J Immunol 125: 2245–2251, 1980
Rich, A., Nordheim, A., and Wang, A. H.-J.: The chemistry and biology of left-handed Z-DNA. Anna Rev Biochem 53: 791–846, 1984
Sanger, F., Nicklen, S., and Coulson, A. R.: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467, 1977
Shimonkevitz, R., Leuscher, B., Cerottini, J.-C., and MacDonald, H. R.: Clonal analysis of cytolytic T lymphocyte-mediated lysis of target cells with inducible antigen expression: correlation between antigen density and requirement for Lyt-2/3 function. J Immunol 135: 892–899, 1985
Shinohara, N. and Sachs, D.: Mouse alloantibodies capable of blocking cytotoxic T cell function. I. Relationship between the antigen reactive with blocking antibodies and the Lyt-2 locus. J Exp Med 150: 432–444, 1979
Sukhatme, V. P., Sizer, K. C., Vollmer, A. C., Hunkapiller, T., and Parties, J. R.: The T cell differentiation antigen Leu-2/T8 is homologous to immunoglobulin and T cell receptor variable regions. Cell 40: 591–597, 1985
Swain, S. L.: Significance of Lyt phenotype: Lyt-2 antibodies block activities of T cells that recognize class I major histocompatibility complex antigens regardless of their function. Proc Natl Acad Sci USA 78: 7101–7105, 1981
Swain, S. L.: T cell subsets and the recognition of MHC class. Immunol Rev 74: 129–142, 1983
Swartz, M. N., Trautner, T. A., and Kornberg, A.: Enzymatic synthesis of deoxyribonucleic acid. XI. Further studies on nearest neighbor base sequences in deoxyribonucleic acids. J Biol Chem 237: 1961–1967, 1962
Weiher, H., Konig, M., and Gruss, P.: Multiple point mutations affecting the simiam virus 40 enhancer. Science 219: 626–631, 1983
Zamoyska, R., Vollmer, A. C., Sizer, K. C., Liaw, C. W., and Parnes, J. R.: Two Lyt-2 polypeptides arise from a single gene by alternative splicing patterns of mRNA. Cell 43: 153–163, 1985
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Youn, H.J., Harriss, J.V. & Gottlieb, P.D. Nucleotide sequence analysis of the C.AKR Lyt-2 agene: structural polymorphism in alleles encoding the Lyt-2.1 T-cell surface alloantigen. Immunogenetics 28, 345–352 (1988). https://doi.org/10.1007/BF00364233
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DOI: https://doi.org/10.1007/BF00364233