Abstract
RFLP analysis of the HLA class II genes DRA, DQA, and DQB was performed in 33 patients with allergic contact eczema to nickel. A significant association with a Taq I HLA-DQA allelic restriction fragment was found. Twenty-two of 33 patients compared to 31 of 100 healthy controls had a 4.5-kb DQA fragment (corrected P value <0.05, relative risk 4.5, and etiologic fraction 0.52). In order to study whether the magnitude of the response in a lymphocyte proliferative assay to nickel sulfate was controlled by HLA class II genes or not, the patients were divided into low, intermediate, and high responders. No significant differences were found in the distribution of Taq I HLA class II allelic patterns between the groups.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Al-Tawil, N. G., Marcusson, J. A., and Möller, E.: Lymphocyte transformation test in patients with nickel sensitivity: an aid to diagnosis. Acta Derm Venereol (Stockh) 61: 511–515, 1981
Al-Tawil, N. G., Marcusson, J. A., and Möller, E.: HLA class 11 restriction of the proliferative T lymphocyte responses to nickel, cobalt and chromium compounds. Tissue Antigens 25: 163–172, 1985
Andersson, M., Böhme, J., Andersson, G., Möller, E., Thorsby, E., Rask, L., and Peterson, P. A.: Genomic hybridization with class 11 transplantation antigen cDNA probes as a complementary technique in tissue typing. Hum Immunol 11: 57–67, 1984
Bengtsson, B. O. and Thomson, G.: Measuring the strength of associations between HLA antigens and diseases. Tissue Antigens 17: 356–363, 1981
Bidwell, J. L.: DNA-RFLP analysis and genotyping of HLA-DR and DQ antigens. Immunol Today 9: 18–23, 1988
Bidwell, J. L., Bidwell, E. A., Laundy, G. J., Klouda, P. T., and Bradley, B. A.: Allogenotypes defined by short DQα and DQβ cDNA probes correlate with and define splits of HLA-DQ serological specificities. Mol Immunol 24: 513–522, 1987
Braathen, L. R., Haavelsrud, O., and Thorsby, E.: HLA antigens in patients with allergic contact sensitivity to nickel. Arch Dermatol Res 275: 355–356, 1983
Böhme, J., Carlsson, B., Wallin, J., Möller, E., Persson, B., Peterson, P. A., and Rask, L.: Only one DQ-β restriction fragment pattern of each DR specificity is associated with insulin-dependent diabetes. J Immunol 137: 941–947, 1986
Carlsson, B., Wallin, J., Bohme, J., and Möller, E.: HLA-DR-DQ haplotypes defined by restriction fragment analysis-correlation to serology. Hum Immunol 20: 95–113, 1987
Cronin, E.: Contact Dermatitis, pp. 338–367, Churchill Livingstone, Edinburgh, 1983
Dumont-Fruytier, M., Van Neste, D., De Bruyere, M., Tennstedt, D., and Lachapelle, J. M.: Nickel contact sensitivity in women and HLA antigens. Arch Dermatol Res 269: 205–208, 1980
Emtestam, L., Marcusson, J. A., and Möller, E.: HLA class II restriction specificity for nickel reactive T lymphocytes. Acta Derm Venereol (Stockh), in press, 1988
Geczy, A. F. and de Week, A. L.: Genetic control of sensitization to structurally unrelated antigens and its relationships to histocompatibility antigens in guinea pigs. Immunology 28: 331–342, 1975
Gustafsson, K., Wiman, K., Emmoth, E., Larhammar, D., Bohme, J., Hyldig-Nielsen, J. J., Ronne, H., Peterson, P. A., and Rask, L.: Mutations and selection in the generation of class II histocompatibility antigen polymorphism. EMBO J 3: 1655–1661, 1984
Hansen, H. E., Menné, T., and Olesen Larsen, S.: HLA antigens in nickel sensitive females. Tissue Antigens 19: 306–310, 1982
Hyldig-Nielsen, J. J., Morling, N., Odum, N., Ryder, L. P., Platz, P., Jakobsen, B., and Svejgaard, A.: Restriction fragment length polymorphism of the HLA-DP subregion and correlations to HLA-DP phenotypes. Proc Natl Acad Sci USA 84: 1644–1688, 1987
Kapoor-Pillarisetti, A., Mowbray, J. F., Brostoff, J., and Cronin E. A.: HLA dependence of sensitivity to nickel and chromium. Tissue Antigens 17: 261–264, 1981
Karvonen, J., Silvennoinen-Kassinen, S., Ilonen, J., Jakkula, H., and Tiilikainen, A.: HLA antigens in nickel allergy. Ann Clin Res 16: 211–212, 1984
Kieffer, M.: Nickel sensitivity: relationship between history and patch test reaction. Contact Dermatitis 5: 398–401, 1979
Larhammar, D., Schenning, L., Gustafsson, K., Wiman, K., Claesson, L., Rask, L., and Peterson, P. A.: Complete amino acid sequence of an HLA-DR antigen-like β chain as predicted from the nucleotide sequence: similarities with immunoglobulin and HLA-A, -B and -C antigens. Proc Natl Acad Sci USA 79: 3687–3691, 1982
Lidén, S., Beckmam, L., Cedergren, K., Göransson, K., and Nyquist, H.: HLA antigens in allergic contact dermatitis. Acta Derm Venereal [Suppl] (Stockh) 79: 53–55, 1978
Lidén, S., Beckman, L., Cedergren, K., Groth, O., Göransson, K., and Wählby, L.: Lack of association between allergic contact dermatitis and HLA antigens of the A and B series. Acta Derm Venereol (Stockh) 61: 155–157, 1981
Menne, T. and Holm, N. V.: Nickel allergy in a female twin population. Int J Dermatol 22: 22–28, 1983
Menné, T. Borgan, O., and Green, A.: Nickel allergy and hand dermatitis in a stratified sample of the Danish female population: an epidemiological study including a statistic appendix. Acta Derm Venereol (Stockh) 62: 35–41, 1982
Peltonen, L.: Nickel sensitivity in the general population. Contact Dermatitis 5: 27–32, 1979
Pirilä, V.: Chamber test versus patch test for epicutaneous testing. Contact Dermatitis 1: 48–52, 1975
Rosenshine, S., Cascino, I., Zeevi, A., Duquesnoy, R. J., and Trucco, M.: DQ a and β RFLP reveals the composition of the DQ molecule recognized by T-cell clones. Immunogenetics 23: 187–196, 1986
Schenning, L., Larhammar, D., Bill, P., Wiman, K., Jonsson, A.-K., Rask, L., and Peterson, P. A.: Both α and β chains of HLA-DC class II histocompatibility antigens display extensive polymorphism in their amino-terminal domains. EMBO J 3: 447–452, 1984
Shultz, L. D. and Bailey, D. W.: Genetic control of contact sensitivity in mice: effect of H-2 and non H-2 loci. Immunogenetics 1: 570–583, 1975
Silvennoinen-Kassinen, J., Ilonen, J., Tiilikainen, A., and Karvonen, J.: No significant association between HLA and nickel contact sensitivity. Tissue Antigens 14: 459–461, 1979
Spielman, R. S., Lee, J., Bodmer, W. F., Bodmer, J. G., and Trowsdale, J.: Six HLA-D region a-chain genes on human chromosome 6: polymorphism and associations of DC a-related sequences with DR types. Proc Natl Acad Sci USA 81: 3461–3465, 1984
Todd, J. A., Bell, J. I., and McDevitt, H. O.: HLA-DQβ gene contributes to susceptibility and resistance to insulin-dependent diabetes mellitus. Nature 329: 599–604, 1987
White, S. I., Friedmann, P. S., and Stratton, A.: HLA antigens and Langerhans cell density in contact dermatitis. Br J Dermatol 115: 447–452, 1986
Woolf, B.: On estimating the relation between blood group and disease. Ann Hum Genet 19: 251–253, 1955
Young, E. and Houwing, R. H.: Patch test results with standard allergens over a decade. Contact Dermatitis 17: 104–107, 1987
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Olerup, O., Emtestam, L. Allergic contact dermatitis to nickel is associated with a Taq I HLA-DQA allelic restriction fragment. Immunogenetics 28, 310–313 (1988). https://doi.org/10.1007/BF00364228
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00364228