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Identification and characterization of a mouse cell surface antigen with alternative molecular forms

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Abstract

We present the characterization of a new mouse cell surface protein, recognized by the 3138-specific monoclonal antibody. The expression of this antigen is predominantly restricted to the hematopoietic and lymphoid tissues: bone marrow, spleen, lymph node, and thymus. Immunoblot analyses show that the 3138 determinant is present on molecules with different apparent relative masses. The 3138 antigen migrates on sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a single band of M r 115 000 for normal nonstimulated spleen cells and thymocytes and as two bands of M r 115 000 and M r 125 000 for bone marrow cells and mitogen-stimulated spleen cells. The multiple sizes of the 3138 antigens (isoforms) found on various cell lines are not due to allelic polymorphism, but instead may reflect the specific cell type or reflect the cell's state of activation or maturation. Results from lectin chromatography and N-glycanase and neuraminidase studies suggest that the 3138 antigen is a heavily sialylated O-linked glycoprotein. The unusual features of this antigen indicate that it may be the mouse homologue of the rat W3/13 antigen and the human leukosialin/sialophorin antigens.

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Abbreviations

Con A:

concanavalin A

Gal:

galactose

Ga1Nac:

N-acetyl galactosamine

Ig:

immunoglobulin

IL-2:

interleukin 2

2-ME:

2-mercapto-ethanol

M r :

relative mass

PAGE:

polyacrylamide gel electrophoresis

PMSF:

phenylmethylsulfonyl fluoride

SDS:

sodium dodecyl sulfate

Th:

T helper

TX-100:

Triton X-100

TTS:

0.3% TX-100, 0.01 M Tris, pH 7.4, 0.15 M NaCl

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Authors and Affiliations

  1. Cancer Center of the University of Rochester Medical Center, 14642, Rochester, NY, USA

    Clare M. Baecher, Anthony J. Infante, Karen L. Semcheski & John G. Frelinger

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  1. Clare M. Baecher
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  2. Anthony J. Infante
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  3. Karen L. Semcheski
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  4. John G. Frelinger
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Baecher, C.M., Infante, A.J., Semcheski, K.L. et al. Identification and characterization of a mouse cell surface antigen with alternative molecular forms. Immunogenetics 28, 295–302 (1988). https://doi.org/10.1007/BF00364226

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  • DOI: https://doi.org/10.1007/BF00364226

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