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Mikropunktionsuntersuchungen über den Einfluß von Aldosteron, Cortison und Dexamethason auf die renale Natriumresorption adrenalektomierter Ratten

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Summary

Using the split oil droplet method the effects of adrenal cortical steroids on impaired renal Na transport have been tested in adrenalectomized white rats.

1. d-aldosterone (dose: 0.125 μg/100 g b.w. intravenously +7.5 μg/100 g b.w. subcutaneously, single dose) after a delay time of about 60 min restored towards normal the local transport capacity for Na of the proximal and distal tubular epithelium.

Equal effects were obtained after repeated administration of d-aldosterone (dose: 7.5 μg TMA-aldosterone/100 g b.w. and 24 hrs, intramuscularly) over 3 to 5 days.

2. Similarily, cortisone in high doses (2.5 mg/100 g b.w. intramuscularly as single dose or repeated administration over 3 to 5 days) normalized Na transport capacity in both segments.

3. Dexamethasone, a synthetic steroid with predominant glucocorticoid activity in a dose equivalent to that of cortisone (50 μg/100 g b.w. and 24 hrs intramuscularly as single dose or repeated administration over 3 to 5 days) did not increase the rate of transtubular net Na transport.

4. From the local Na transport capacity and proximal transit time measured with lissamin green fractional NaCl and fluid reabsorption in the proximal convolution has been estimated. Aldosterone, which did not influence proximal transit time, increased fractional reabsorption above control values and above values obtained from adrenalectomized rats without hormone substitution. Fractional reabsorption was normalized by cortisone or was decreased by dexamethasone.

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Authors and Affiliations

  1. Physiologisches Institut der Freien Universität Berlin, Germany

    M. Wiederholt, H. Stolte, J. P. Brecht & K. Hierholzer

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  1. M. Wiederholt
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  2. H. Stolte
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  3. J. P. Brecht
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  4. K. Hierholzer
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Mit Unterstützung durch die Deutsche Forschungsgemeinschaft.

Auszugsweise vorgetragen auf der 30. Tagung der Deutschen Physiologischen Gesellschaft, Bad Nauheim 1965.

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Wiederholt, M., Stolte, H., Brecht, J.P. et al. Mikropunktionsuntersuchungen über den Einfluß von Aldosteron, Cortison und Dexamethason auf die renale Natriumresorption adrenalektomierter Ratten. Pflügers Archiv 292, 316–333 (1966). https://doi.org/10.1007/BF00363200

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