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Nachweis höchster Carboanhydrase-Aktivitäten im Epithel der Speicheldrüsengänge

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Summary

The distribution of carbonic anhydrase in salivary glands of rats, mice, rabbits and cats has been analysed by a histochemical method. Enzymic activity was generally found in the striated ducts, the intraglandular excretory ducts and in the serous gland cells. The extraglandular main ducts also contain some carbonic anhydrase. The enrichment of carbonic anhydrase in the basal striations of the ducts is very similar to the enzyme localisation in the distal tubules of the kidney. This supports the view that in salivary glands Na+ is reabsorbed by the ducts; on the other hand it lends support to the suggestion that cations are excreted by these ducts. Furthermore the histochemical test probably indicates the structures, where in the ducts salivary bicarbonate is made from CO2.

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Literatur

  • Brusilow, S. W., and R. E. Cooke: Role of parotid ducts in secretion of hypotonic saliva. Amer. J. Physiol. 196, 831–834 (1959).

    Google Scholar 

  • Burgen, A. S. V.: In A. S. V. Burgen, and N. G. Emmelin: Physiology of the salivary glands. Monographs of the Physiological Society, No 8, p. 146, 147. London: Edward Arnold Ltd 1961.

    Google Scholar 

  • — and P. Seeman: The role of the salivary duct system in the formation of saliva. Canad. J. Biochem. 36, 119–143 (1958).

    Google Scholar 

  • — and K. G. Terroux: Influxes sites in the duct system of the dog parotid gland. J. Physiol. (Lond.) 161, 393–413 (1962).

    Google Scholar 

  • — K. G. Terroux, and E. Gonder: The sites of transfer of sodium, potassium and iodide in the parotis duct system of the dog. Canad. J. Biochem. 37, 359–370 (1959).

    Google Scholar 

  • Denton, D. A.: The effect of Na depletion on the Na: K ratio of the parotid saliva of the sheep. J. Physiol. (Lond.) 131, 516–525 (1956).

    Google Scholar 

  • — and I. R. McDonald: The effect of rapid change of Na balance on the Na/K ratio of the parotid saliva of Na depleted sheep. J. Physiol. (Lond.) 133, 37–38P (1956).

    Google Scholar 

  • Goding, J. R., and D. A. Denton: Adrenal cortex and the parotid secretion of sodium-depleted sheep. Science 123, 986–987 (1956).

    Google Scholar 

  • Goor, H. van: Carbonic anhydrase, its properties, distribution and significance for carbon dioxide transport. Enzymologia 13, 73–164 (1948/49).

    Google Scholar 

  • Häusler, G.: Histochemischer Nachweis von Carboanhydrase. Verh. Anat. Ges. 54. Vers. 1957. Anat. Anz. 104, Ergänzungs-Heft 391–395 (1958).

    Google Scholar 

  • — Zur Technik und Spezifität des histochemischen Carboanhydrasenachweises im Modellversuch und in Gewebsschnitten von Rattennieren. Z. Zellforsch., Abt. Histochem. 1, 29–47 (1958).

    Google Scholar 

  • Henriques, B. L.: Acinar-duct transport sites for Na+ and K+ in dog submaxillary salivary gland. Amer. J. Physiol. 201, 935–938 (1961).

    Google Scholar 

  • Junqeira, L. C. U., and F. F. De Moraes: Comparative aspects of the vertebrate maior salivary gland biology. In: Sekretion und Exkretion, S. 36–47. Hrsg. von K. E. Wolfarth-Bottermann. Berlin, Heidelberg, New York: Springer 1965.

    Google Scholar 

  • Langley, L. L., and R. S. Brown: Stop flow analysis of ionic transfer in the dog parotid gland. Amer. J. Physiol. 199, 59–62 (1960).

    Google Scholar 

  • Leder, O.: Nachweis höchster Carboanhydrase-Aktivitäten im Epithel der Speicheldrüsen-Gänge. Pflügers Arch. ges. Physiol. 281, 54–55 (1964).

    Google Scholar 

  • — Die Verteilung von Carboanhydrase im Rattenáuge. Pflügers Arch. ges. Physiol. 287, 351–356 (1966).

    Google Scholar 

  • — u. R. Groh: Histochemische Untersuchungen über die Carboanhydrase-Aktivität der Rattenplacenta. Pflügers Arch. ges. Physiol. 285, 19–25 (1965).

    Google Scholar 

  • McClanahan, H. H., and W. R. Amberson: Bicarbonate elimination through the salivary glands under nervous and chemical stimulation. J. Pharmacol. exp. Ther. 53, 189–197 (1935).

    Google Scholar 

  • Martinez, J. R., H. Holzgreve u. K. J. Ullrich: Mikropunktionsuntersuchungen an der Glandula submaxillaris der Ratte. Pflügers Arch. ges. Physiol. 279, R 17 (1964).

  • Morris, G. C. R., and G. W. Swayne: Histochemical studies of carbonic anhydrase in the salivary glands of rabbits. J. Physiol. (Lond.) 171, 5P-6P (1964).

    Google Scholar 

  • Sand, H. F.: The carbonic acid content of saliva and its role in the formation of dental calculus. Thesis, Universität Oslo 1949.

  • — Source of bicarbonate of saliva. J. appl. Physiol. 4, 66–78 (1951).

    Google Scholar 

  • Schneyer, C. A., and L. H. Schneyer: Secretion by salivary glands deficient in acini. Amer. J. Physiol. 201, 939–942 (1961).

    Google Scholar 

  • Schögel, E., and J. A. Young: Micropuncture and perfusion investigation of sodium and potassium transport in the rat submaxillary gland. J. Physiol. (Lond.) 183, 73P-75P (1966).

    Google Scholar 

  • Thaysen, J. H., N. A. Thorn, and I. L. Schwartz: Excretion of sodium, potassium, chloride and carbon dioxide in human parotid saliva. Amer. J. Physiol. 178, 155–159 (1954).

    Google Scholar 

  • Wechsler, A.: The secretion of bicarbonate in saliva M. Sc. Thesis, Mc Gill University 1959; zit. nach A. S. V. Burgen and N. G. Emmelin: Physiology of the salivary glands (Monographs of the Physiological Society, No 8), p. 146. London: Edward Arnold Ltd. 1961.

  • White, A. G., P. S. Entmacher, G. Rubin, and L. Leiter: Physiological and pharmacological regulation of human salivary electrolyte concentrations with a discussion of electrolyte concentrations of some other exocrine secretions. J. clin. Invest. 34, 246–255 (1955).

    Google Scholar 

  • Yoshimura, H., T. Inone, Y. Imai, and F. Yoshimura: Studies on mechanism of salivary secretion. Jap. J. Physiol. 12, 467–483 (1962).

    Google Scholar 

  • — H. Iwasaki, T. Nishikawa, and S. Matsumoto: Role of carbonic anhydrase in the bicarbonate excretion from salivary glands and mechanism of ionic excretion. Jap. J. Physiol. 9, 106–123 (1959).

    Google Scholar 

  • — M. Miyoshi, S. Matsumoto, F. Fujimoto, and Y. Yamamoto: Reflection of salt concentrations of blood upon those of saliva. Jap. J. Physiol. 13, 523–540 (1963).

    Google Scholar 

  • Young, J. A., E. Frömter, and E. Schögel: Electrolyte fluxes and potential differences in the main excretory duct of the rat sub-maxillary gland. Pflügers Arch. ges. Physiol. 289, R 81 (1966).

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Authors and Affiliations

  1. Physiologisches Institut der Universität Freiburg i. Br., Germany

    Ortwin Leder & Peter Tritschler

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  1. Ortwin Leder
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  2. Peter Tritschler
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Additional information

Eine vorläufige Mitteilung der Ergebnisse ist bereits während der 29. Tagung (Herbsttagung) der Deutschen Physiologischen Gesellschaft in Tübingen vom 29. 9.–3. 10. 1964 erfolgt [vgl. Pflügers Arch. ges. Physiol. 281, 54–55 (1964)].

Wesentliche Teile der vorliegenden Arbeit wurden von Herrn P. Tritschler der Medizinischen Fakultät der Universität Freiburg i. Br. als Dissertation vorgelegt.

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Leder, O., Tritschler, P. Nachweis höchster Carboanhydrase-Aktivitäten im Epithel der Speicheldrüsengänge. Pflügers Archiv 292, 229–238 (1966). https://doi.org/10.1007/BF00362738

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  • DOI: https://doi.org/10.1007/BF00362738

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