Aktionspotentiale der Ganglienzelle des Auerbachschen Plexus im Kaninchendünndarm

Summary

Action potentials of ganglion cells in the Auerbach's plexus of the rabbit's intestine were recorded by means of silver or platinum-iridium electrodes.

When the silver electrode was inserted through the serosal membrane and the longitudinal muscle at a distinct point of the isolated rabbit's intestine canal, characteristic spike bursts were obtained in accordance with phases of rhythmic segmentations, namely with intervals of 10–30 sec. Each spike had a duration of about 20 msec and was followed by a long positive wave. The amplitude of each spike was generally much larger than that which was recorded extracellularly from the longitudinal or circular muscle layer. Afterwards the localization of ganglion cells at the reading point of the electrode was ascertained by methylene blue staining.

In other cases the platinum-iridium electrode was laid on the ganglion cell of the Auerbach's plexus, which adhered to the stripped longitudinal muscle layer. The characteristic spike potential recorded hereby showed also intervals of 10–30 sec.

The action potential from the ganglion cell of the Auerbach's plexus was to be observed in Tyrode solution generally for 1 hr after the beginning of soaking, disappeared promptly when the oxygen supply was interrupted and reappeared when oxygen was supplied again. By application of hexamethonium solution (in conc. of 4·10⁻⁵ g/cm³) the electrical activity of the Auerbach's plexus stopped in about 30 sec.