Abstract
Cultures were made of kidney cells of male Rhesus monkey and a karyological analysis of these cells was carried out at various times after plating using the chromosome banding method of Seabringht (1972), in order to study the segregation phenomena which take place in cell cultures in vitro through multipolar mitoses and to identify segregating cells. — Several segregating cells were found: haploid cells, diploid cells with two X chromosomes and triploid cells which were strictly euploid. Polyploidization-segregation cycles in the cell cultures and their mechanism and chronological sequence were analyzed. — On the basis of these results it was suggested that the genome is organized in haploid sets capable of segregating as units and of producing strictly euploid segregating daughter cells.
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Rizzoni, M., Palitti, F. & Perticone, P. Euploid segregation through multipolar mitosis in mammalian cell cultures. Chromosoma 45, 151–162 (1974). https://doi.org/10.1007/BF00362308
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DOI: https://doi.org/10.1007/BF00362308