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A gene tightly linked to CEN6 is important for growth of Saccharomyces cerevisiae

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Summary

Transcriptional analysis of the region flanking the left boundary of the centromere of chromosome VI revealed the presence of a gene immediately adjacent to CEN6. The transcription of the gene is directed toward the centromere, and nucleotide sequence analysis showed that the coding region terminates only 50 bp away from CEN6. Our results extend to chromosome VI the observation that centromere-flanking regions of S. cerevisiae are transcriptionally active. Disruption of the coding region of the gene showed that its product, whilst not essential for cell viability, is important for normal cell growth. The gene has been termed DEG1 (DEpressed Growth rate). Comparison of the deduced amino acid sequence of DEG1 with a protein sequence databank revealed homology with the enzyme tRNA pseudouridine synthase I of E. coli.

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Author notes

  1. Manuela Solinas

    Present address: Istituto di Genetica Biochimica ed Evoluzionistica del CNR, via Abbiategrasso 207, I-27100, Pavia, Italy

Authors and Affiliations

  1. Dipartimento di Genetica e di Biologia dei Microrganismi, Università di Milano, via Celoria 26, I-20133, Milano, Italy

    Maria Luisa Agostoni Carbone & Lucia Panzeri

  2. Dipartimento di Genetica e Microbiologia, Università di Pavia, vía S. Epifanio 14, I-27100, Pavia, Italy

    Silvio Sora

Authors
  1. Maria Luisa Agostoni Carbone
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  2. Manuela Solinas
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  3. Silvio Sora
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  4. Lucia Panzeri
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Communicated by G. Simchen

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Agostoni Carbone, M.L., Solinas, M., Sora, S. et al. A gene tightly linked to CEN6 is important for growth of Saccharomyces cerevisiae . Curr Genet 19, 1–8 (1991). https://doi.org/10.1007/BF00362080

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  • DOI: https://doi.org/10.1007/BF00362080

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