Abstract
A porcine glucosephosphate isomeraseprocessed pseudogene has been isolated and sequenced. The pseudogene has several base substitutions as well as an insertion and deletions, and is 83% homologous to the corresponding cDNA. It contains an intervening sequence of 565 bp, is truncated at the 3′ end, and is flanked by direct repeats of seven nucleotides. Fluorescent in situ hybridization to porcine metaphase chromosomes localized the processed pseudogene to Chromosome (Chr) 1q1.6-1.7. A (GT)14(AT)15 microsatellite was detected close to the processed pseudogene.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Breathnach, R., Benoist, C., O'Hare, K., Gannon, F., Chambon, P. (1978). Ovalbumin gene: evidence for a leader sequence in mRNA and DNA sequences at the exon-intron boundaries. Proc. Natl. Acad. Sci. USA 75, 4853–4857.
Chaput, M., Claes, V., Portetelle, D., Cludts, I., Cravador, A., Burny, A., Gras, H., Tartar, A. (1988). The neurotrophic factor neuroleukin is 90% homologous with phosphohexose isomerase. Nature 332, 454–455.
Claes, V., Taquet, A.N., Kettmann, R., Burny, A. (1990). Sequence analysis of the pig phosphoglucose isomerase gene promotor region. Biochim. Biophys. Acta 1087, 339–340.
Davies, W., Harbitz, I., Hauge, J.G. (1987). A partial cDNA clone for porcine glucosephosphate isomerase: isolation, characterization and use in detection of restriction fragment length polymorphisms. Anim. Genet. 18, 233–240.
Davies, W., Harbitz I., Fries, R., Stranzinger, G., Hauge, J.G. (1988). Porcine malignant hyperthermia carrier detection and chromosomal assignment using a linked probe. Anim. Genet. 19, 203–212.
Devereux, J., Haeberli, P., Smithies, O. (1984). A comprehensive set of sequence analysis programs for the Vax. Nucleic Acids Res. 12, 387–395.
Dyer, M.R., Gay, N.J., Walker, J.E. (1989). DNA sequence of a bovine gene and of two related pseudogenes of the proteolipid subunit of mitochondrial ATP synthase. Biochem. J. 260, 249–258.
Ellegren, H., Johansson, M., Chowdhary, B.P., Marklund, S., Ruyter, D., Marklund, L., Nielsen, P.B. Edfors-Lilja, I., Gustavsson, I., Juneja, R.K., Andersson, L. (1993). Assignment of twenty porcine markers to the porcine linkage map. Genomics, in press.
Faik, P., Walker, J.I.H., Redmill, A.A.M., Morgan, M.J. (1988). Mouse glucose-6-phosphate isomerase and neuroleukin have identical 3′ sequences. Nature 332, 455–457.
Harbitz, I., Kristensen, T., Bosnes, M., Kran, S., Davies, W. (1992). DNA sequence of the skeletal muscle calcium release channel cDNA and verification of the Arg615→Cys615 mutation, associated with porcine malignant hyperthermia, in Norwegian Landrace pigs. Anim. Genet. 23, 395–402.
Kohne, D.E. (1970). Evolution of higher-organism DNA. Q. Rev. Biophys. 33, 327–375.
Novacek, M.J. (1992). Mammalian phylogeny: shaking the tree. Nature 356, 121–125.
Perler, F., Efstratiadis, A., Lomedico, P., Gilbert, W., Kolodner, R., Dodgson, J. (1980). The evolution of genes: the chicken preproinsulin gene. Cell 20, 555–566.
Popescu, N.C., Amsbaugh S.C., Swan, D.C., Di Paolo, P.A. (1985). Induction of chromosome banding by trypsin/EDTA for gene mapping by in situ hybridization. Cytogenet. Cell Genet. 39, 73–74.
Vanin, E.F. (1985). Processed pseudogenes: characteristics and evolution. Annu. Rev. Genet. 19, 253–272.
Walker, J.I., Faik, P., Morgan, M.J. (1990). Characterization of the 5′ end of the gene for human glucose phosphate isomerase (GPI). Genomics 7, 638–643.
Weiner, A.M., Deininger, P.L., Efstratiadis, A. (1986). Nonviral retroposons: genes, pseudogenes, and transposable elements generated by the reverse flow of genetic information. Annu. Rev. Biochem. 55, 631–661.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Harbitz, I., Chowdhary, B.P., Kran, S. et al. Characterization of a porcine glucosephosphate isomerase-processed pseudogene at Chromosome 1q1.6-1.7. Mammalian Genome 4, 589–592 (1993). https://doi.org/10.1007/BF00361390
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00361390