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Studies on the histones of the ciliate Stylonychia mytilus

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Abstract

Histones were extracted from pure macronuclei and maoronuolear anlagen and were analyzed by different electrophoretic techniques and by amino acid analysis. Fractionation of the histones on SDS-gels showed that the histone fractions of the macronucleus and the macronuclear anlagen are identical in their molecular weights. Comparison with calf thymus histone fractions showed considerable similarities in the molecular weights. Analysis by polyacrylamide-urea gel electrophoresis and by amino acid analysis showed quantitative differences in some histone fractions between these two types of nuclei.

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References

  • Allfrey, V. G.: Functional and metabolic aspects of DNA-associated proteins. In: Histones and nucleohistones (D. M. P. Phillips, ed.). New York: Plenum Publishing Corporation 1971

    Google Scholar 

  • Allfrey, V. G., Mirsky, A. E.: Mechanisms of synthesis and control of protein and ribonucleic acid synthesis in the cell nucleus. Cold Spr. Harb. Symp. quant. Biol. 28, 247–262 (1963)

    Google Scholar 

  • Ammermann, D.: Cytologische und genetische Untersuchungen an dem Ciliaten Stylonychia mytilus Ehrenberg. Arch. Protistenk. 108, 109–152 (1965)

    Google Scholar 

  • Ammermann, D.: Synthese und Abbau der Nucleinsäuren während der Entwicklung des Makronukleus von Stylonychia mytilus (Protozoa, Ciliata). Chromosoma (Berl.) 25, 107–120 (1968)

    Google Scholar 

  • Ammermann, D.: The micronucleus of the ciliate Stylonychia mytilus. Its nucleic acid synthesis and its function. Exp. Cell Res. 61, 6–12 (1970)

    Google Scholar 

  • Ammermann, D.: Morphology and development of the macronuclei of the ciliates Stylonychia mytilus and Euplotes aediculatus. Chromosoma (Berl.) 33, 209–237 (1971)

    Google Scholar 

  • Ammermann, D., Steinbrück, G. X., Berger, L. v., Hennig, W.: The development of the macronucleus in the ciliated protozoan Stylonychia mytilus. Chromosoma (Berl.) 45, 401–429 (1974)

    Google Scholar 

  • Asao, T.: Behaviour of histones and cytoplasmic basic proteins during embryogenesis of the Japanese newt, Triturus pyrrhogaster. Exp. Cell Res. 58, 243–252 (1969)

    Google Scholar 

  • Benttinnen, L. C., Comb, D. G.: Early and late histones during sea urchin development. J. molec. Biol. 57, 355–358 (1971)

    Google Scholar 

  • Butler, J. A. V.: Fractionation and characteristics of histones. In: The Nucleohistones (J. Bonner and P. Ts'o, eds.) San Francisco: Holden-Day 1964

    Google Scholar 

  • Delange, R. J., Fambrough, D. M., Smith, E. L., Bonner, J.: Calf and pea histone IV. III. Complete amino acid sequence of pea seedling histone IV; comparison with the homologous calf thymus histone. J. biol. Chem. 244, 5669 (1969)

    Google Scholar 

  • Evans, L. E., Ozaki, H.: Nuclear histones of unfertilized sea urchin eggs. Exp. Cell Res. 79, 228–231 (2973)

    Google Scholar 

  • Fambrough, D. M., Fuijmura, F., Bonner, J.: Quantitative distribution of histone components in the pea plant. Biochemistry 7, 575–584 (1968)

    Google Scholar 

  • Gorovsky, M. A.: Studies on nuclear strcuture and function in Tetrahymena pyriformis. III. Comparison of the histones of macro- and micronuclei by quantitative polyacrylamide gel electrophoresis. J. Cell Biol. 47, 631–636 (1970)

    Google Scholar 

  • Hnilica, L. S.: The structure and biological function of histones. Cleveland: C. R. C. Press 1972

    Google Scholar 

  • Isaacks, R. E., Santos, B. G.: Studies on nuclei of Paramecium aurelia. 2. Amino acid composition and electrophoretic properties of the chromosomal basic proteins. J. Protozool. 20, 482–489 (1973)

    Google Scholar 

  • Johns, E. W.: Studies on histones. 7. Preparative methods for histone fractions from calf thymus. Biochem. J. 92, 55–59 (1964)

    Google Scholar 

  • Johns, E. W.: A method for the selective extraction of histone fractions f 2(a)1 and f2(a)2 from calf thymus deoxyribonucleoprotein. Biochem. J. 105, 611–614 (1967)

    Google Scholar 

  • Johns, E. W.: The preparations and characteristics of histones. In: Histones and nucleohistones (D. M. P. Phillips, ed.). New York: Plenum Publishing Corporation 1971

    Google Scholar 

  • Kaye, J. S., McMaster-Kaye, R.: Histones of spermatogonous cells in the house cricket. Chromosoma (Berl.) 46, 387–419 (1974)

    Google Scholar 

  • Kornberg, R. D.: Chromatin structure: A repeating unit of histones and DNA. Science 184, 868–871 (1974)

    Google Scholar 

  • Kornberg, R. D., Thomas, J. O.: Chromatin structure: Oligomers of the histones. Science 184, 865–868 (1974)

    Google Scholar 

  • Lipps, H. J., Sapra, G. R., Ammermann, D.: The histones of the ciliated protozoan Stylonychia mytilus. Chromosoma (Berl.) 45, 273–280 (1974)

    Google Scholar 

  • Mirsky, A. E., Silvermann, B.: Effects of selective extraction of histones on template activities of chromatin by use of exogenous DNA and RNA polymerases. Proc. nat. Acad. Sci. (Wash.) 70, 1973–1975 (1973)

    Google Scholar 

  • Pallotta, D., Berlowitz, L., Rodriguez, L.: Histones of genetically active and inactive chromatin in mealy bugs. Exp. Cell Res. 60, 474 (1970)

    Google Scholar 

  • Panyim, S., Chalkley, R.: High resolution acrylamide gel electrophoresis of histones. Arch. Biochem. Biophys. 130, 337–346 (1969a)

    Google Scholar 

  • Panyim, S., Chalkley, R.: Heterogenity of histones. I. A quantitative analysis of calf histone in very long polyacrylamide gels. Biochemistry 8, 3972–3979 (1969b)

    Google Scholar 

  • Panyim, S., Chalkley, R.: The molecular weights of vertebrate histones exploiting a modified sodium dodecyl sulfate electrophoretic method. J. biol. Chem. 246, 7557–7560 (1971)

    Google Scholar 

  • Pogo, B. G. T., Allfrey, V. G., Mirsky, A. E.: RNA synthesis and histone acetylation during the course of gene activation in Lymphocytes. Proc. nat. Acad. Sci. (Wash.) 55, 805–812 (1966)

    Google Scholar 

  • Pogo, B. G. T., Pogo, A. O., Allfrey, V. G., Mirsky, A. E.: Changing patterns of histone acetylation and RNA synthesis in regeneration of the liver. Proc. nat. Acad. Sci. (Wash.) 59, 1337–1344 (1968)

    Google Scholar 

  • Tessier, A., Pallotta, D.: Analysis of basic proteins during spermatogenesis in the cricket Acheta domestica. Exp. Cell Res. 82, 103–110 (1973)

    Google Scholar 

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Lipps, H.J., Hantke, K.G. Studies on the histones of the ciliate Stylonychia mytilus . Chromosoma 49, 309–320 (1975). https://doi.org/10.1007/BF00361073

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