Abstract
The erythromycin resistance gene (Emr) from Campylobacter jejuni ABA94 plasmid DNA was cloned into the pUC18 vector and then expressed in Escherichia coli. The location of the Emr determinant on the chimeric plasmid was determined by restriction endonuclease mapping within a 0.8-kb EcoRI fragment. This fragment then hybridized to the 78-kb plasmid DNA but not to the 3.3-or 12.6-kb plasmid DNA of Campylobacter jejuni ABA94. Emr in Campylobacter jejuni is therefore probably plasmid-mediated.
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The authors are with the Department of Genetics and Cellular Biology, University of Malaya, 59100 Kuala Lumpur, Malaysia
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Son, R., Ansary, A. Cloning and expression of the erythromycin resistance determinant from Campylobacter jejuni in Escherichia coli . World Journal of Microbiology & Biotechnology 11, 685–686 (1995). https://doi.org/10.1007/BF00361018
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DOI: https://doi.org/10.1007/BF00361018