Abstract
A sporulating, aerobic Bacillus sp., isolated from Chimanimani hot springs, Zimbabwe, produced endoglucanase when cultured on medium with initial pH between 5.0 and 9.0 and at 30 to 60°C. Optimal production of endoglucanase was at pH 6.0. The enzyme was constitutively produced when the organism was cultured on starch, cellobiose, carboxymethylcellulose, sucrose, glucose, galactose, Avicel, lactose, mannose or maltose.
Similar content being viewed by others
References
AliS.M. & AkhandA.A. 1992 Cellulase from Trichoderma isolate. Journal of Basic Microbiology 32, 259–268.
AuK.S. & ChanK-Y. 1986 Carboxymethycellulase production by Bacillus subtilis. Microbios 48, 93–108.
CowanT.S. & SteelJ.K. 1993 Characters of gram-positive bacteria. In Manual for the Identification of Medical Bacteria, eds BarrowG.I. & FelthamR.K.A. pp. 50–93. Cambridge: Cambridge University Press.
DegryseE., GlansdorffN. & PierardA. 1978. A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus. Archives of Microbiology 117, 189–196.
DhillonN., ChhibberS., SaxenaM., PajniS. & VadehraD.V. 1985 A constitutive endoglucanase (CMCase) from Bacillus licheniformis-1. Biotechnology Letters 7, 695–697.
GhanghasS.G. & WilsonD.B. 1987. Expression of a Thermomonospora fusca cellulase gene in Streptomyces lividans and Bacillus subtilis. Applied and Environmental Microbiology 53, 1470–1475.
GhoseT.K. 1987. Measurement of cellulase activities. Pure and Applied Chemistry 59, 257–268.
GilkesN.R., KiburnD.G., MilerR.C. & WarrenR.A.J. 1991 Bacterial cellulases. Bioresource Technology 36, 21–35.
HarmovaM., BielyP. & VrasnskaM. 1986 Specificity of cellulase and β-xylanase induction in Trichoderma reesei QM 9414. Archives of Microbiology 144, 307–311.
MalekM.A., ChowdhuryN.A., YoussoufQ.M. & ChoudhuryN. 1988 Bacterial cellulases and saccharification of lignocellulosic materials. Enzyme and Microbial Technology 10, 750–753.
MullingsR. & ParishJ.H. 1984. Mesophilic aerobic Gram negative cellulose degrading bacteria from aquatic habitats and soils. Journal of Applied Bacteriology 57, 455–468.
WaldronG. & EveleighD.E. 1986. Saccharification of cellulosics by Microbiospora bispora. Applied Microbiology and Biotechnology 4, 487–492.
Additional information
The authors are with the Fermentation and Food Group, Department of Biochemistry, University of Zimbabwe, Box MP 167, Mount Pleasant, Harare, Zimbabwe
Rights and permissions
About this article
Cite this article
Zvauya, R., Zvidzai, C.J. Constitutive production of endoglucanase by a Bacillus sp. isolated from a Zimbabwean hot spring. World Journal of Microbiology & Biotechnology 11, 658–660 (1995). https://doi.org/10.1007/BF00361011
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00361011