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Optimization of single-cell-protein production from cassava starch using Schwanniomyces castellii

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Abstract

Schwanniomyces castellii B5285 grew faster and produced greater biomass and higher protein yield than either S. alluvius ATCC 26074 or S. alluvius 81Y when these amylolytic yeasts were grown with 2% (w/v) cassava starch as sole C source. With 0.5% (w/v) glutamate as N source, S. castellii reached 7.12 g cell dry mass/l, with a protein yield of 6.4 g/100 g starch. The optimal agitation speed, aeration rate and pH for growth of this yeast in a fermenter were 400 rev/min, 1.67 vol./vol.min. and 5.0, respectively. Tween 80 at 0.1% increased cell dry mass to 8.90 g/l, cell yield to 44 g/100 g starch and protein yield to 7.4 g/100 g starch.

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The authors are with the Department of industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai 90110, Thailand

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Hongpattarakere, T., H-Kittikun, A. Optimization of single-cell-protein production from cassava starch using Schwanniomyces castellii . World Journal of Microbiology & Biotechnology 11, 607–609 (1995). https://doi.org/10.1007/BF00360999

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  • DOI: https://doi.org/10.1007/BF00360999

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