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Evaluation de la matière organique vivante dans les eaux marines par la mesure de l'adénosine triphosphate

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Abstract

Quantitative determination of living organic matter (micro-organisms) in the ocean were made using the adenosine triphosphate (ATP) assay. Strehler and Totter's (1952) method was used. A simple apparatus recorded the photons emitted from the very beginning of the photochemical reaction; the assay is specific for ATP but enzymes present in Photynus pyralis suspension may create errors. The sea-water ATP values in Marseilles Bay range between 2.10-2 and 80.10-2 mg per m3. An offshore profile of the Mediterranean Sea, near Toulon, gives values around 10-3 mg/m3. Protein assays have been made on the same samples. The differences existing between certain values in the series are attributed to inert particles.

Résumé

  1. 1.

    La matière organique vivante d'une masse d'eau peut être connue par la mesure de l'adénosine triphosphate (ATP).

  2. 2.

    Un dispositif simple permet l'enregistrement de l'émission lumineuse produite par action de l'ATP sur le complexe luciférine-luciférase.

  3. 3.

    Les valeurs observées dans le Golfe de Marseille sont comprises entre 2.10-2 et 80.10-2 mg/m3, alors qu'en profondeur, au large de Toulon, elles sont voisines de 10-3 mg/m3.

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Author notes

  1. R. Daumas

    Present address: Station Marine d'Endoume et Centre d'Océanographie, Marseille, France

  2. M. Flala

    Present address: Laboratoire ARAGO, 66, Banyulssur-Mer, France

Authors and Affiliations

  1. Station Marine d'Endoume et Centre d'Océanographie, Marseille, France

    M. Flala

Authors
  1. R. Daumas
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  2. M. Flala
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Communicated by J. M. Peres, Marseille

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Daumas, R., Flala, M. Evaluation de la matière organique vivante dans les eaux marines par la mesure de l'adénosine triphosphate. Marine Biology 3, 243–246 (1969). https://doi.org/10.1007/BF00360957

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  • DOI: https://doi.org/10.1007/BF00360957

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