Abstract
Extracts from wheat embryos have been used to study the incorporation of amino acids into TCA insoluble products using hepatic mRNA fractions. The properties of this system are described and compared to the incorporation obtained with poly U and rabbit globin mRNA. SDS-acrylamide gel analysis showed that the major polypeptide synthesized with globin mRNA co-migrates with rabbit globin (15 500 daltons). Rat liver products were numerous, with molecular weights from less than 10000 to greater than 65000 daltons. The KCl concentration for maximum incorporation into TCA precipitable polypeptides with hepatic mRNA was not the optimum KCl concentration for synthesis of complete products.
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Astell, C.R., Ganoza, M.C. Translation of hepatic mRNA in extracts from wheat germ embryos. Molecular Biology Reports 1, 483–491 (1974). https://doi.org/10.1007/BF00360676
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DOI: https://doi.org/10.1007/BF00360676