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Renaturation of denatured DNA in NaI gradients

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Abstract

We show that denatured DNA from Tetrahymena mitochondria or phage T7, partially renatures during centrifugation in NaI equilibrium density gradients. This makes these gradients unsuitable for the analysis of single-stranded complementary nucleic acids, especially if their complexity and mole percent G+C are low.

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Abbreviations

H-strand:

heavy strand

L-strand:

light strand

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Author notes

  1. R. A. Clegg

    Present address: Department of Biochemistry, Medical Sciences Institute, The University, DD1 4HN, Dundee, Great Britain

Authors and Affiliations

  1. Section for Medical Enzymology, Laboratory of Biochemistry, University of Amsterdam, Amsterdam, The Netherlands

    R. A. Clegg & P. Borst

Authors
  1. R. A. Clegg
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  2. P. Borst
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Clegg, R.A., Borst, P. Renaturation of denatured DNA in NaI gradients. Molecular Biology Reports 1, 477–482 (1974). https://doi.org/10.1007/BF00360675

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  • DOI: https://doi.org/10.1007/BF00360675

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