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Formyl-met-tRNA supMetinff , a questionable initiator tRNA in eukaryotic systems

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Abstract

Met-tRNA supMetinff is a much more efficient initiator in cell-free eukaryotic protein synthesis than the formylated species. Chemically and enzymatically formylated Met-tRNA supMetinff exhibit the same activity.

In contrast acetyl Met-tRNA supMetinff is unable to initiate polypeptides in vitro.

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References

  1. Smith, A. E. and Marcker, K. A., Nature (London) 226, 607 (1970).

    Google Scholar 

  2. Brown, J. C. and Smith, A. E., Nature (London) 226, 610 (1970).

    Google Scholar 

  3. Jackson, R. and Hunter, T., Nature (London) 227, 672 (1970).

    Google Scholar 

  4. Housman, D., Jacobs-Lorena, M., RajBhandary, U. L., and Lodish, H. F., Nature (London) 227, 913 (1970).

    Google Scholar 

  5. Öberg, B. F. and Shatkin, A. J., Proc. Nat. Acad. Sci. U.S. 69, 3589 (1972).

    Google Scholar 

  6. Morrison, T. G. and Lodish, H. F., Proc. Nat. Acad. Sci. U.S. 70, 315 (1973).

    Google Scholar 

  7. Jacobs-Lorena, M. and Baglioni, C., Mol. Biol. Rep. 1, 113 (1973).

    Google Scholar 

  8. Smith, A. E. and Wigle, D. T., Eur. J. Biochem. 35, 566 (1973).

    Google Scholar 

  9. Smith, A. E. and Marcker, K. A., J. Mol. Biol. 38, 241 (1968).

    Google Scholar 

  10. Strous, G. J. A. M., Berns, A. J. M., VanWestreenen, H., and Bloemendal, H., Eur. J. Biochem. 30, 48 (1972).

    Google Scholar 

  11. Strous, G. J. A. M., VanWestreenen, H., and Bloemendal, H., Eur. J. Biochem. 38, 79 (1973).

    Google Scholar 

  12. Marcker, K. A. and Sanger, F., J. Mol. Biol. 8, 835 (1964).

    Google Scholar 

  13. Schofield, P. and Zamecnik, P. C., Biochim. Biophys. Acta 155, 410 (1968).

    Google Scholar 

  14. Strous, G. J. A. M., VanWestreenen, H., Van derLogt, J., and Bloemendal, H., Biochim. Biophys. Acta 353, 89 (1974).

    Google Scholar 

Download references

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Author notes

  1. G. J. A. M. Strous

    Present address: Dept. of Histology and Cell Biology, State University, Nic. Beetsstraat 22, Utrecht, The Netherlands

Authors and Affiliations

  1. Dept. of Biochemistry, University of Nijmegen, Nijmegen, The Netherlands

    G. J. A. M. Strous, Th. Bollen & H. Bloemendal

Authors
  1. G. J. A. M. Strous
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  2. Th. Bollen
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  3. H. Bloemendal
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Strous, G.J.A.M., Bollen, T. & Bloemendal, H. Formyl-met-tRNA supMetinff , a questionable initiator tRNA in eukaryotic systems. Molecular Biology Reports 1, 471–475 (1974). https://doi.org/10.1007/BF00360674

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  • DOI: https://doi.org/10.1007/BF00360674

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