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Translation of pure feather keratin mRNA in a wheat embryo cell-free system

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Abstract

Highly purified feather keratin mRNA, prepared by dissociation of mRNP particles in Na dodecyl sulphate, was translated in a wheat embryo cell-free system with similar efficiency to rabbit globin mRNA and RNA purified from cucumber mosaic virus. The only detectable products of translation of the keratin mRNA were keratin chains, which were identical to native keratin chains as judged by several different criteria. These results support previous conclusions that the keratin mRNA can be obtained in a pure state.

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Authors and Affiliations

  1. Dept. of Biochemistry, University of Adelaide, 5001, Adelaide, South Australia, Australia

    David J. Kemp, Mark W. Schwinghamer & George E. Rogers

Authors
  1. David J. Kemp
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  2. Mark W. Schwinghamer
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  3. George E. Rogers
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Kemp, D.J., Schwinghamer, M.W. & Rogers, G.E. Translation of pure feather keratin mRNA in a wheat embryo cell-free system. Molecular Biology Reports 1, 441–446 (1974). https://doi.org/10.1007/BF00360669

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  • DOI: https://doi.org/10.1007/BF00360669

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