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Porcine SINE-associated microsatellite markers: evidence for new artiodactyl SINEs

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Abstract

Approximately 24% (170/710) of porcine (dG-dT)n·(dC-dA)n microsatellites isolated in our laboratory are associated with a previously described porcine Short Interdispersed Element (SINE) termed PRE-1 SINE. Another 5.6% (40/710) of the microsatellites were adjacent to two previously unidentified SINE sequences, which we have designated ARE-1P (Artiodactyl Repetitive Element-1 Porcine) and ARE-2P. The ARE repeats were also found in bovine microsatellite and genomic sequences in the GenBank database. Genotypic information was obtained from 68.9% of primers where at least one primer sequence was obtained from the PRE-1 SINE and 66.6% of primer pairs designed from the ARE SINEs. The use of primers derived from SINEs significantly increases the number of primer pairs available for genetic linkage studies in swine.

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Alexander, L.J., Rohrer, G.A., Stone, R.T. et al. Porcine SINE-associated microsatellite markers: evidence for new artiodactyl SINEs. Mammalian Genome 6, 464–468 (1995). https://doi.org/10.1007/BF00360655

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