Abstract
The genetic map positions of 12 simple sequence repeat (SSR) markers spanning mouse Chromosome (Chr) 16 were determined relative to reference markers on that chromosome. Interval mapping data were obtained with a panel of DNAs from two intersubspecific backcrosses. All but one of the markers were typed by use of nonradioactive polymerase chain reaction (PCR) products analyzed on agarose gels. The marker order was determined to be Prm-1, D16Mit9, Igl-1, D16Mit29, D16Mit1/D16Mit2, Smst, D16Mit4, D16Mit11, Gap43, D16Mit14, D16Mit30, D16Mit5, Pit-1, D16Mit27, D16H21S16 (formerly D21S16h), D16Mit19, App, D16Mit7, Sod-1. Two of these markers mapped to the known human Chr 21 (HSA21)/Chr 16 conserved linkage group. Nine additional SSR markers could not be typed because they were not polymorophic (four markers), did not amplify MOLD/Rk DNA (three markers), or failed to give PCR products under a range of conditions (two markers). A subset of the most robust SSRs provide a useful marker set for the analysis of previously unmapped crosses.
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References
Dietrich, W., Katz, H., Lincoln, S.E., Shin, H.-S. Friedman, J., Dracopoli, N., Lander, E.S. (1992). A genetic map of the mouse suitable for typing intraspecific crosses. Genetics 131, 423–447.
Gregor, P., Reeves, R.H., Jabs, E.W., Wang, X., Dackowski, W., Rochelle, J.M., Brown, R.H., Haines, J.L., O'Hara, B.F., Uhl, G.R., Seldin, M.F. (1993). Chromosomal localization of glutamate receptor genes: relationship to familial amyotrophic lateral sclerosis and other neurological disorders of mice and man. Proc. Natl. Acad. Sci. USA 90, 3053–3057.
Love, J.M., Knight, A.M., McAleer, M.A., Todd, J.A. (1990). Toward construction of a high resolution map of the mouse genome using PCR-analyzed microsatellites. Nucleic Acids Res. 18, 4123–4130.
Miller, R.D., Ozaki, J.H., Riblet, R. (1993). The mouse severe combined immune deficiency (scid) mutation is closely linked to the B cell specific developmental genes VpreB and λ5. Genomics, in press.
O'Brien, S.J. (1991). Mammalian genome mapping: lessons and prospects. Curr. Opin. Genet. Dev. 1, 105–111.
Reeves, R.H., Crowley, M.R., Moseley, W.S., Seldin, M.F. (1991). Comparison of interspecific to intersubspecific backcrosses demonstrates species and sex differences in recombination frequency on mouse chromosome 16. Mammalian Genome, 1, 158–164.
Reeves, R.H. Irving, N.G., Miller, R.D., (1993). Mouse Chromosome 16. Mammalian Genome 4 (Suppl), in press.
Weber, J.L., May, P.E., (1989). Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. Am. J. Hum. Genet. 44, 388–396.
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Irving, N.G., Citron, M.P. & Reeves, R.H. The positions of 12 simple sequence repeat markers relative to reference loci on mouse Chromosome 16. Mammalian Genome 4, 364–367 (1993). https://doi.org/10.1007/BF00360586
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DOI: https://doi.org/10.1007/BF00360586