Abstract
Type-1-protein phosphatase (PP-1) activity is reduced in skeletal muscle from human subjects with insulin resistance (Kida et al. 1990). This reduced phosphatase activity probably leads to the abnormal insulin action for glucose storage observed in insulin-resistant subjects. In the present study, a human homolog of rat liver PP-1γ1 cDNA was isolated from human skeletal muscle. The nucleotide sequence contains a 957-nucleotide open reading frame encoding an amino acid sequence identical to that encoded by rat liver PP-1γ1 cDNA. Northern blot analysis shows PP-1γ1-specific mRNA is expressed in human heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. PP-1γ1 was localized to human Chromosome 12.
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Alessi, D.R., Street, A.J., Cohen, P., Cohen, P.T.W. (1993). Inhibitor-2 functions like a chaperone to fold three expressed isoforms of mammalian protein phosphatase-1 into a conformation with the specificity and regulatory properties of the native enzyme. Eur. J. Biochem. 213, 1055–1066.
Aviv, H., Leder, P. (1972). Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc. Natl. Acad. Sci. USA 69, 1408–1412.
Bai, G.E., Zhang, Z., Jahanshah, A., Deans-Zirattu, S.A., Lee, E.Y.C. (1988). Molecular cloning of a cDNA for the catalytic subunit of rabbit muscle phosphorylase phosphatase. FASEB J. 2, 3010–3016.
Barker, H.M., Jones, T.A., da Cruz e Silva, E.F., Spurr, N.K., Sheer, D., Cohen, P.T.W. (1990). Localization of the gene encoding a type 1 protein phosphatase catalytic subunit to human chromosome band 11q13. Genomics 7, 159–166.
Berndt, N., Campbell, D.G., Caudwell, F.B., Cohen, P, da Cruz e Silva, E.F., da Cruz e Silva, O.B., Cohen, P.T.W. (1987). Isolation and sequence analysis of a cDNA clone encoding a type-1 protein phosphatase catalytic subunit: homology with protein phosphatase 2A. FEBS Lett. 233, 340–346.
Bogardus, C., Lillioja, S., Stone, K., Mott, D.M. (1984). Correlation between muscle glycogen synthase activity and in vivo insulin action in man. J. Clin. Invest. 85, 476–481.
Bollen, M., Stalmans, W. (1992). The structure, role and regulation of type-1 protein phosphatases. Crit. Rev. Biochem. Mol. Biol. 27, 227–281.
Braun, T., Bober, E., Buschhausen-Denker, G., Kohtz, S., Grzeschik, K.H., Arnold, H.H. (1989). Differential expression of myogenic determination genes in muscle cells: possible autoactivation by the myf gene products. EMBO J. 8, 3617–3625.
Chomczynski, P., Sacchi, N. (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156–159.
Cohen, P. (1989). The structure and regulation of protein phosphatase. Annu. Rev. Biochem. 58, 453–508.
Cohen, P.T.W. (1988). Two isoforms of protein phosphatase 1 may be produced from the same gene. FEBS Lett. 232, 17–23.
Feinberg, A.P., Vogelstein, B. (1983). A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132, 6–13.
Kida, Y., Esposito-Del Puente, A., Bogardus, C., Mott, D.M. (1990). Insulin resistance is associated with reduced fasting and insulin-stimulated glycogen synthase phosphatase activity in human skeletal muscle. J. Clin. Invest. 85, 476–481.
Kida, Y., Raz, I., Maeda, R., Nyomba, B.L., Stone, K., Bogardus, C., Sommercorn, J., Mott, D.M. (1992). Defective insulin response of phosphorylase phosphatase in insulin-resistant humans. J. Clin. Invest. 89, 610–617.
Pearson, W.R., Lipman, D.J. (1988). Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. USA 85, 2444–2448.
Proudfoot, N.J., Brownlee, G.G. (1976). 3′ non-coding region sequences in eukaryotic messenger RNA. Nature 263, 211–214.
Sanger, F., Niklen, S., Coulson, A.R. (1977). DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463–5467.
Sasaki, K., Shima, H., Kitagawa, Y., Irino, S., Sugimura, T., Nagao, M. (1990). Identification of members of the protein phosphatase 1 gene family in the rat and enhanced expression of protein phosphatase 1α gene in rat hepatocellular carcinomas. Jpn. J. Cancer Res. 81, 1272–1280.
Shulman, G.I., Rothman, D.L., Jue, T., Stein, P., DeFronzo, R.A., Shulman, R.G. (1990). Quantitation of muscle glycogen synthesis in normal subjects and subjects with non-insulin-dependent diabetes by 13C nuclear magnetic resonance spectroscopy. N. Engl. J. Med. 322, 223–228.
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Norman, S.A., Mott, D.M. Molecular cloning and chromosomal localization of a human skeletal muscle PP-1γ1 cDNA. Mammalian Genome 5, 41–45 (1994). https://doi.org/10.1007/BF00360567
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DOI: https://doi.org/10.1007/BF00360567