Abstract
The fresh water polyp Hydra can reproduce asexually by forming buds. These buds separate from the parent animal due to the development of foot tissue in a belt-like region and the formation of a constriction basal to that region. A single pulse treatment with activators of protein kinase C, including 1,2-dioctanoyl-rac-glycerol and 12-o-tetradecanoylphorbol-13-acetate, and inhibitors of various protein kinases, including staurosporine, H-7 and genistein, interfered with foot and constriction formation. The buds did not separate. Therewith, branched animals were formed, some of which bore a lateral foot patch. Simultaneous treatments with an activator and inhibitor led to a higher amount of branched animals than treatments with one of these agents alone. Based on the different specificities of the activators and inhibitors used we propose that activation of a protein kinase C and/or inhibition of a probably non-C-type protein kinase interfere with the decrease of positional value at the bud's base, a process necessary to initiate the pattern forming system leading to foot formation.
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Correspondence to: F. Perez
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Pérez, F., Berking, S. Protein kinase modulators interfere with bud formation in Hydra vulgaris . Roux's Arch Dev Biol 203, 284–289 (1994). https://doi.org/10.1007/BF00360524
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DOI: https://doi.org/10.1007/BF00360524