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Localization of the B-hordein locus on barley chromosomes using fluorescence in situ hybridization

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Abstract

The B-hordein gene family consists of at least 13 genes that appear to be clustered in two regions on the barley chromosome 1H (5). Using fluorescence in situ hybridization techniques, we have localized the B-hordein locus (Hor2) to the distal end of the short arm of chromosome 1H (5), corresponding to the genetically determined position, 91% distal on the short arm. This result is based on appropriate barley lines (i.e. the two cultivars Igri and Betzes, the telotrisomic and ditelotetrasomic lines 1HS and the translocation line T21), on two experimental approaches and on a signal frequency of between 20% and 60% on metaphase chromosomes.

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Authors and Affiliations

  1. Botanisches Institut der Ludwig-Maximilians-Universität München, Menzingerstrasse 67, 80638, München, Germany

    H. Lehfer, W. Busch, R. Martin & R. G. Herrmann

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  1. H. Lehfer
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  2. W. Busch
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  3. R. Martin
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  4. R. G. Herrmann
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Communicated by: D. Schweizer

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Lehfer, H., Busch, W., Martin, R. et al. Localization of the B-hordein locus on barley chromosomes using fluorescence in situ hybridization. Chromosoma 102, 428–432 (1993). https://doi.org/10.1007/BF00360408

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  • DOI: https://doi.org/10.1007/BF00360408

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