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Hybridization of mRNA and pre-mRNA with the sequences forming double-stranded structures in pre-mRNA

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Abstract

About 25% of the double-stranded sequences isolated from pre-mRNA are able to hybridize, after melting, with either mRNA or non-melted pre-mRNA. The retention of one branch of pre-mRNA hairpin in mRNA was suggested. It was also found that in addition to the hairpin-like structures comprising about 3% of the total sequences another 15% of the pre-mRNA sequences can form double-stranded structures upon annealing over a broad interval of Cot values.

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Authors and Affiliations

  1. Institute of Molecular Biology, Acad. of Sciences of the U.S.S.R., Moscow, Russia

    A. P. Ryskov, S. A. Limborska & G. P. Georgiev

Authors
  1. A. P. Ryskov
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  2. S. A. Limborska
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  3. G. P. Georgiev
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Ryskov, A.P., Limborska, S.A. & Georgiev, G.P. Hybridization of mRNA and pre-mRNA with the sequences forming double-stranded structures in pre-mRNA. Molecular Biology Reports 1, 215–219 (1973). https://doi.org/10.1007/BF00357644

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  • DOI: https://doi.org/10.1007/BF00357644

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