Abstract
The gene for the β-A subunit of inhibin (INHBA) was assigned to bovine syntenic group U13 by bovine x rodent hybrid somatic cells and the polymerase chain reaction (PCR). A 482-bp PCR fragment was used to clone a 37-kb cosmid. This cosmid was assigned to bovine Chromosome (Chr) 4 (BTA 4) by fluorescence in situ hybridization (FISH). This is the first assignment of a U13 marker to a bovine chromosome. A restriction fragment length polymorphism (RFLP) was detected with PstI within the INHBA cosmid.
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Barton, D.E., Yang-Feng, T.L., Mason, A.J., Seeburg, P.H., and Francke, U. (1987). INHA, INHBA and INHBB, the loci for three subunits of inhibin mapped in mouse and man. Cytogenet. Cell Genet. 46, 578.
Barton, D.E., Yang-Feng, T.L., Mason, A.J., Seeburg, P.H., and Francke, U. (1989). Mapping of genes for inhibin subunits alpha, beta A and beta B on human and mouse chromosomes and studies of jsd mice. Genomics 5, 91–99.
Blin, N. and Stafford, D.W. (1976). A general method for isolation of high molecular weight DNA from eukaryotes. Nucleic Acids Res. 3, 2303–2308.
Broad, T.E., Ansari, H.A., Burkin, D.J., Cambridge, L., Davidson, B.P., Hones, C., Lewis, P.E., McNabb, L.R., Maher, D.W., Malcolm, A.A., Pearce, P.D., Phua, S.H., and Wood, N.J. (1993). Twenty new loci mapped to the ovine genome. Anim. Genet. 23 Suppl., 94 (Abstract).
Brubacher, M.G., Siewertsen, M.A., Billingsley, G.D., Benger, J.C., and Cox, D.W. (1990). A genetic linkage map of human chromosome 14 and homology with the mouse. Am. J. Hum. Genet. 47 Suppl., A175 (Abstract).
Chevalet, C. and Corpet, F. (1986). Statistical decision rules concerning synteny or independence between markers. Cytogenet. Cell Genet. 43, 132–139.
Copeland, N.G. and Jenkins, N.A. (1991). Development and applications of a molecular genetic linkage map of the mouse genome. Trends Genet. 7, 113–118.
Esch, F.S., Shimasaki, S., Cooksey, K., Mercado, M., Mason, A.J., Ying, S.-Y., Ueno, N., and Ling, N. (1987). Complementary deoxyribonucleic acid (cDNA) cloning and DNA sequence analysis of rat ovarian inhibins. Mol. Endocrinol. 1, 388–396.
Forage, R.G., Ring, J.M., Brown, R.W., McInerney, B.V., Cobon, G.S., Gregson, R.P., Robertson, D.M., Morgan, J.F., Hearn, M.T., Findlay, J.K., Wettenhall, R.E.H., Burger, J.G., and Krester, D.M. (1986). Cloning and sequence analysis of cDNA species coding for the two subunits of inhibin from bovine follicular fluid. Proc. Natl. Acad. Sci. USA 83, 3091–3095.
Gallagher, D.S., Jr. and Womack, J.E. (1992). Chromosome conservation in the Bovidae. J. Hered. 83, 287–298.
Hayes, H., Petit, E., Lemieux, N., and Dutrillaux, B. (1992). Chromosomal localization of the ovine beta-casein gene by nonisotopic in situ hybridization and R-banding. Cytogenet. Cell Genet. 61, 286–288.
ISCNDA 1989 (1990). International system for cytogenetic nomenclature of domestic animals, Di Berardino, D., Hayes, H., Fries, R., and Long, S., eds. Cytogenet. Cell Genet. 53, 65–79.
Johnson, G.D. and Araujo, G.M. de C.N. (1981). A simple method of reducing the fading of immunofluorescence during microscopy. J. Immunol. Methods 43, 349–350.
Levan, G., Szpirer, J., Szpirer, C., Klinga, K., Hanson, C., and Islam, M.Q. (1991). The gene map of the Norway rat (Rattus norvegicus) and comparative mapping with mouse and man. Genomics 10, 699–718.
Ling, N., Ying, S.A., Ueno, N., Shimaski, S., Esch, R., Hotta, M., and Guillemin, R. (1986). Pituitary FSH is released by a heterodimer of the β-subunits from two forms of inhibin. Nature 321, 779–782.
Mason, A.J., Hayflick, J.S., Ling, N., Esch, F., Ueno, N., Ying, S.-Y., Guillemin, R., Niall, H.D., and Seeburg, P. (1985). Complementary DNA sequences of ovarian follicular fluid inhibin show precursor structure and homology with transforming growth factor-β. Nature 318, 659–663.
Mason, A.J., Niall, H.D., and Seeburg, P. (1986). Structure of two human ovarian inhibins. Biochem. Biophys. Res. Commun. 135, 957–964.
Nadeau, J.H. (1989). Maps of linkage and synteny homologies between mouse and man. Trends Genet. 5, 81–86.
Reading Conference 1980 (1980). Proceedings of the first international conference for the standardization of banded karyotypes of domestic animals. Reading, England (1976). Ford, C.E., Pollock, D.L., Gustavsson, I. (eds). Hereditas 92, 145–162.
Rivier, C., Rivier, J., and Vale, W. (1986). Inhibin-mediated feedback control of follicle-stimulating hormone secretion in the female rat. Science 234, 205–208.
Rodgers, R.J. (1991). Cloning of the inhibin/activin beta B subunit gene from the Booroola Merino sheep. J. Mol. Endocrinol. 6, 87–93.
Steele, M.R. and Georges, M. (1991). Generation of bovine multisite haplotypes using random cosmid clones. Genomics 10, 889–904.
Threadgill, D.W. and Womack, J.E. (1990). Genomic analysis of the major bovine milk protein genes. Nucleic Acids Res. 18, 6935–6942.
Vale, W., Spiess, J., Rivier, C., and Rivier, J. (1981). Characterization of a 41-residue ovine hypothalamic peptide that stimulates secretion of corticotropin and β-endorphin. Science 213, 1394–1397.
Vale, W., Rivier, J., Vaughan, J., McClintock, R., Corrigan, A., Woo, W., Karr, D., and Spiess, J. (1986). Purification and characterization of an FSH releasing protein from porcine ovarian follicular fluid. Nature 321, 776–779.
Womack, J.E. (1990). Gene mapping in the cow. In: Advances in Veterinary Science and Comparative Medicine: Domestic Animal Cytogenetics, R.A. McFeely, ed. (San Diego, Calif.: Academic Press, Inc.), pp. 251–271.
Womack, J.E. and Moll, Y.D. (1986). Gene map of the cow: conservation of linkage with mouse and man. J. Hered. 77, 2–7.
Womack, J.E., Bolsch, S., and Fries, R. (1993). Gene map of the cow (Bos taurus) 2N=60. In: Genetic Maps, S.J. O'Brien, ed. (Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press), in press.
Woodruff, T.K., D'Agostino, J.-B., Schwartz, N.B., and Mayo, K.E. (1988). Dynamic changes in inhibin messenger RNAs in rat ovarian follicles during the reproductive cycle. Science 239, 1296–1299.
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Neibergs, H.L., Gallagher, D.S., Georges, M. et al. Physical mapping of inhibin β-A in domestic cattle. Mammalian Genome 4, 328–332 (1993). https://doi.org/10.1007/BF00357092
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DOI: https://doi.org/10.1007/BF00357092