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Chromosome banding in Amphibia

XVI. High-resolution replication banding patterns in Xenopus laevis

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Abstract

High-resolution replication banding patterns were induced in prometaphase and prophase chromosomes of Xenopus laevis by treating kidney cell lines with 5-bromodeoxyuridine (BrdU) and deoxythymidine (dT) in succession. Up to 650 early and late replicating bands per haploid karyotype were demonstrated in the very long prophase chromosomes. This permits an exact identification of all chromosome pairs of X. laevis. Late replicating heterochromatin was located by analysing the time sequence of replication throughout the second half of S-phase. Neither heteromorphic sex chromosomes nor sex chromosome-specific replication bands were demonstrated in the heterogametic ZW females of X. laevis. A detailed examination of the BrdU/dT-labelled prometaphases and prophases revealed that the X. laevis chromosomes can be arranged in groups of four (quartets), most of which show conspicuous similarities in length, centromere position, and replication pattern. This is interpreted as further evidence for an ancient allotetraploid origin of X. laevis.

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Authors and Affiliations

  1. Department of Human Genetics, University of Würzburg, Biozentrum, Am Hubland, W-8700, Würzburg, Federal Republic of Germany

    M. Schmid & C. Steinlein

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  1. M. Schmid
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  2. C. Steinlein
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This paper is dedicated to Prof. Wolfgang Engel on the occasion of his 50th birthday

by H.C. Macgregor

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Schmid, M., Steinlein, C. Chromosome banding in Amphibia. Chromosoma 101, 123–132 (1991). https://doi.org/10.1007/BF00357062

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  • DOI: https://doi.org/10.1007/BF00357062

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