Abstract
The Xist sequence has several characteristics that make it a potential candidate for the X-inactivation center. To investigate the role of Xist and adjacent sequences lying within the X-inactivation center candidate region, a 460-kb region surrounding the murine Xist sequence has been arrayed in lambda contigs with a combination of IRS-PCR-based hybridization and YAC fragmentation. The orientation of the Xist sequence in relation to the telomere and centromere of the X Chromosome (Chr) has been established with this contig and shown to be inverted compared to that in human.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Amar, L.C., Dandolo, L., Hanauer, A., Ryder Cook A., Arnaud, D., Mandel, J.-L. Avner, P. (1988). Conservation and reorganization of loci on the mammalian X chromosome: a molecular framework for the identification of homologous subchromosomal regions in man and mouse. Genomics 2, 220–230.
Arveiler, B., Porteous, D.J. (1992). Distribution of Alu and L1 repeats in human YAC recombinants. Mamm. Genome 3, 661–668.
Borsani, B., Tonlorenzi, R., Simmler, M.-C., Dandolo, L., Arnaud, D., Capra, V., Grompe, M., Pizzuti, A., Muzni, D., Lawrence, C., Willard, H.F., Avner, P., Ballabio, A. (1991). Characterization of a murine gene expressed from the inactive X chromosome. Nature 351, 325–329.
Boyle, A.L., Ballard, S.G., Ward, D.C. (1990). Differential distribution of long and short interspaced element sequences in the mouse genome: chromosome karyotyping by fluorescence in situ hybridization. Proc. Natl. Acad. Sci. USA 87, 7757–7761.
Brockdorff, N., Ashworth, A., Kay, G.F., Cooper, P., Smith, S., McCabe, V.M., Norris, D.P., Penny, G.D., Patel, D., Rastan, S. (1991). Conservation of position and exclusive expression of mouse Xist from the inactive X chromosome. Nature 351, 329–331.
Brockdorff, N., Ashworth, A., Kay, G.F., McCabe, V.M., Norris, D.P., Cooper, P.J., Swift, S., Rashan, S. (1992) The product of the mouse Xist gene is a 15 kb inactive x-specific, transcript containing to conserved ORF and located in the nucleus. Cell 71, 515–526.
Brown, C.J., Ballabio, A., Rupert, J.L., Lafrenièe, R.G., Grompe, M., Tonlorenzi, R., Willard, H.F. (1991). A gene from the region of the human X inactivation centre is expressed exclusively from the inactive X chromosome. Nature 349, 38–44.
Brown, C.J., Hendrich, B.D., Rupert, J.L., Lafreniere, R.G., Xing, Y., Lawrence, J., Willard, H.F. (1992). The human XIST gene: analysis of a 17 kb inactive X-specific RNA that contains conserved repeats and is highly localized within the nucleus. Cell 71, 527–542.
Cattanach, B.M., Rasberry, C., Evans, E.P., Dandolo, L., Simmler, M.C., Avner, P. (1991). Genetic and molecular evidence of an X-chromosome deletion spanning the tabby (Ta) and testicular feminization (Tfm) loci in the mouse. Cytogenet. Cell Genet. 56, 137–143.
Church, G.M., Gilbert, W. (1984). Genomic sequencing. Proc. Natl. Acad. Sci. USA 81, 1991–1995.
Colleaux, L., Rougeulle, C., Avner, P., Dujon, B., (1993). Rapid physical mapping of YAC inserts by random integration of I-Sce I sites. Hum Mol. Genet. 2, 265–271.
Evans, G. (1991). Combinatoric strategies for genome mapping. BioEssays 13, 39–44.
Faust, C.J., Levinson, B., Gitschier, J., Herman, G. (1992). Extension of the physical map in the region of the mouse X chromosome homologous to human Xq28 and identification of an exception to conserved linkage. Genomics 13, 1289–1295.
Hastie, N.D. (1989). Highly repeated DNA families in the genome of Mus musculus. In Genetics Variants and Strains of the Laboratory Mouse. M.F. Lyon, A.G. Searle, eds. Oxford: Oxford University), pp. 559–573.
Heard, E., Simmler, M.C., Larin, Z., Rougeulle, C., Courtier, B., Lehrach, H., Avner, P. (1993). Physical mapping and YAC contig analysis of the region surrounding Xist on the mouse X chromosome. Genomics 15, 559–569.
Larin, Z., Monaco, A.P., Lehrach, H. (1991). Yeast artificial chromosome libraries containing large inserts from mouse and human DNA. Proc. Natl. Acad. sci. USA 88, 4123–4127.
Leppig, K.A., Brown, C.J., Bressler, S.L., Gustashaw, K., Pogon, R.A., Willard, H.F., Discheche, C.M. (1993). Mapping of the distal boundary of the X-inactivation center in a reaaranged X chromosome from a female expressing Xist. Hum. Mol. Genet. 2, 883–887.
Nelson, D.L. Weis, J.H., Przyborski, M.-J., Mulligan, R.C., Seidman, J.G., Housman, D.E. (1984). Metaphase chromosome transfer of introduced selectable markers. J. Mol. Appl. Genet. 2, 563–577.
Pavan, W.J., Hieter, P., Reeves, R.H. (1990). Generation of deletion derivatives by targeted transformation of human-derived yeast artificial chromosomes. Proc. Natl. Acad. Sci. USA 87, 1300–1304.
Pieretti, M., Tonlorenzi, R., Ballabio, A. (1991). Rapid assembly of λ phage contigs within YAC clones. Nucleic Acids Res. 19, 2795–2796.
Rastan, S. (1983). Non-random X-chromosome inactivation in mouse X-autosome translocation embryos—location of the inactivation center. J. Embryol. Exp. Morphol. 78, 1–22.
Rastan, S., Robertson, E.J. (1985). X-chromosome deletions in embryoderived (EK) cell lines associated with lack of X-chromosome inactivation. J. Embryol. Exp. Morphol. 90, 379–388.
Russell, L.B. (1983). X-autosome translocations in the mouse. Their characterization and use as tools to investigate gene inactivation and gene action. In Cytogenetics of the Mammalian X Chromosome, Part A: Basic Mechanisms of X Chromosome Behaviour. Sandberg A.A., ed. (New York: A.R. Liss) pp. 205–250.
Simmler, M.-C., Cox, R.D., Avner, P. (1991). Adaptation of interspersed repetitive sequence polymerase chain reaction to the isolation of mouse DNA probes from somatic cell hybrids on a hamster background. Genomics 10, 770–778.
Simmler, M.-C., Cattanach, B.M., Rasberry, C., Rougeulle, C., Avner, P. (1993). Mapping the murine Xce locus with (CA)n repeats. Mamm. Genome 4, 523–530.
Thierry, A., Dujon, B. (1992). Nested chromosomal fragmentation in yeast using the meganuclease I-Sce I: a new method for physical mapping of eukaryotic genome. Nucleic Acid Res. 20, 5625–5631.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rougeulle, C., Colleaux, L., Dujon, B. et al. Generation and characterization of an ordered lambda clone array for the 460-kb region surrounding the murine Xist sequence. Mammalian Genome 5, 416–423 (1994). https://doi.org/10.1007/BF00357001
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00357001