Skip to main content
SpringerLink
Log in

A directed alteration of ribonuclease specificity. Hydrolysis of polyribonucleotides containing modified cytosine bases

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

The action of ribonucleases on poly and oligoribonucleotides containing cytosine bases modified by methoxyamine and bisulphite was examined. Resistance of phosphodiester bonds in (Cp) n Xp (where n ≥ 1 and X stands for A, G or U) to T2 RNase hydrolysis was observed if substrates were modified chemically. The phenomenon formed the basis for isolation of (Cp) n Xp blocks as an additional tool in sequence investigations. After modification of cytosine pancreatic RNase was unable to hydrolyse (Cp) n Up blocks. Therefore the specificity of pyrimidyl RNase may be narrowed to ‘uridyl RNase’.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

\(\begin{array}{*{20}c} {\user2{ **}} \\ \user2{C} \\ \end{array}\) :

cytidine modified with methoxyamine and bisulphite (5, 6-dihydro-6-sulpho-N4-methoxycytidine)

\(\begin{array}{*{20}c} {\user2{ *}} \\ \user2{C} \\ \end{array}\) :

cytidine modified with methoxyamine (N4-methoxycytidine)

References

  1. Kochetkov, N. K. and Budovsky, E. I., Progr. Nucl. Acids Res. 9, 403 (1969).

    Google Scholar 

  2. Lee, J. C., Ho, N. W. Y., and Gilham, P. T., Biochim. Biophys. Acta 95, 503 (1965).

    Google Scholar 

  3. Ho, H. W. Y., Uchida, T., Egamy, F., and Gilham, P. T., Cold Spring Harbor Symp. 34, 647 (1969).

    Google Scholar 

  4. Avdonina, T. A. and Kisselev, L. L., Mol. Biol. Reports 1, 283 (1974).

    Google Scholar 

  5. Budowsky, E. I., Sverdlov, E. D., and Monastyrskaya, G. S., FEBS Letters 25, 201 (1972).

    Google Scholar 

  6. Scheinker, V. S. and Kisselev, L. L., Molekularn. Biologiya 9, 105 (1975).

    Google Scholar 

  7. Tomlinson, R. V. and Tener, G. M., J. Am. Chem. Soc. 84, 2644 (1962).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Molecular Biology, Academy of Sciences, 117312, Moscow, USSR

    Alexander M. Mazo, Vladimir S. Scheinker & Lev. L. Kisselev

Authors
  1. Alexander M. Mazo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Vladimir S. Scheinker
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lev. L. Kisselev
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mazo, A.M., Scheinker, V.S. & Kisselev, L.L. A directed alteration of ribonuclease specificity. Hydrolysis of polyribonucleotides containing modified cytosine bases. Molecular Biology Reports 2, 233–239 (1975). https://doi.org/10.1007/BF00356993

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00356993

Keywords

Search

Navigation