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Studies on holocellulose and alpha-cellulose from spruce wood using cryo-ultramicrotomy

Part I: Structural changes of the fibre walls during delignification and alkali extraction

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Summary

Cryo-ultramicrotomy was applied to holocellulose and alpha-cellulose from spruce wood (Picea abies Karst.) for a light and electron microscopical study of the removal of lignin during chlorite delignification and the changes in swelling during delignification and alkali extraction. The swelling state of the fibre walls during each stage of treatment was well preserved, and distinct differences could be observed. Staining with uranyl acetate brought out the fine structure of the fibre walls down to the range of elementary fibrils.

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Authors and Affiliations

  1. Institut für Holzforschung der Universität München, Winzererstr. 45, D-8000, München 40, Federal Republic of Germany

    Manfred Stoll & Dietrich Fengel

Authors
  1. Manfred Stoll
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  2. Dietrich Fengel
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Additional information

Submitted by Deutsche Forschungsgemeinschaft. The assistance of Dr. D. Grosser at the light microscope and of Miss U. Schwarz with the chemical analysis is greatly appreciated.

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Stoll, M., Fengel, D. Studies on holocellulose and alpha-cellulose from spruce wood using cryo-ultramicrotomy. Wood Sci. Technol. 11, 265–274 (1977). https://doi.org/10.1007/BF00356925

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  • DOI: https://doi.org/10.1007/BF00356925

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