Abstract
The use of integrated mapping strategies involving bacterial, yeast, and rodent cells as hosts simplifies the construction of maps, which combine long-range order, high resolution, and easy access to the cloned DNA. Radiation-fusion hybrids offer a specially powerful long-range mapping system for human chromosomes. We describe here techniques for establishing a radiation-fusion hybrid map of Chromsome (Chr) 21 q and its integration with local information on YAC and cosmid positions.
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Kumlien, J., Labella, T., Zehetner, G. et al. Efficient identification and regional positioning of YAC and cosmid clones to human Chromosome 21 by radiation fusion hybrids. Mammalian Genome 5, 365–371 (1994). https://doi.org/10.1007/BF00356556
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DOI: https://doi.org/10.1007/BF00356556