Abstract
A bovine genomic library enriched for DNA fragments bearing poly(dC-dA).poly(dG-dT) sequences was prepared and screened. Twenty-four clones bearing microsatellites were subject to sequence analysis and marker development as appropriate. Three of the 24 clones had microsatellites that were not evaluated for marker development owing to presence of a satellite element in two cases and limited repeat length in the third. Of the remaining 21 clones, all but one yielded polymorphic microsatellites. All but two of the polymorphic markers could be assigned to chromosomal locations by either linkage analysis or on the basis of X-linked inheritance as determined by heterozygosity limited to females. An unexpected clustering of markers was observed as 4 of the 20 polymorphic microsatellites mapped to a region of less than approximately 30 cM on Chromosome (Chr) 19, and four markers displayed X-linked inheritance.
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Barendse, W., Armitage, S.M., Kossarek, L.M., Shalom, A., Kirkpatrick, B.W., Ryan, A.M., Clayton, D., Li, L., Neibergs, H.L., Zhang, N., Grosse, W.M., Weiss, J., Creighton, P., McCarthy, F., Ron, M., Teale, A.J., Fries, R., McGraw, R.A., Moore, S.S., Georges, M., Soller, M., Womack, J.E., Hetzel, D.J.S. (1994). A genetic linkage map of the bovine genome. Nature Genet. 6, 227–235.
Dietrich, W.F., Miller, J.C., Steen, R.G., Merchant, M., Damron, D., Nahf, R., Gross, A., Joyce, D.C., Wessel, M., Dredge, R.D., Marquis, A., Stein, L.D., Goodman, N., Page, D.C., Lander, E.S. (1994). A genetic map of the mouse with 4,006 simple sequence length polymorphisms. Nature Genet. 7, 220–245.
Green, P. (1992). Construction and comparison of chromosome 21 radiation hybrid and linkage maps using CRI-MAP. Cytogenet. Cell Genet. 59, 122–124.
Hillier, L., Green, P. (1991). OSP: a computer program for choosing PCR and DNA sequencing primers. PCR methods Appl. 1, 124–128.
Kirkpatrick, B.W., Hart, G.L. (1994). Conformation polymorphisms and targeted marker development. Anim. Genet. 25, 77–82.
Kirkpatrick, B.W., Bradshaw, M., Dentine, M.R., Barendse, W. (1995). Polymorphic bovine microsatellites UWCA25, UWCA26 and UWCA28. Anim. Genet. 26, 59–60.
Ma, R.Z., Beever, J.E., Da, L., Green, C.A., Russ, I., Park, C., Heyen, D.W., Everts, R.E., Teal, A.J., Kemp, S.J., Hines, H.C., Guerin, G., Lewin, H.A. (1995). A male linkage map of the cattle (Bos taurus) genome. J. Hered., in press.
Moore, S.S., Byrne, K., Berger, K.T., Barendse, W., McCarthy, F., Womack, J.E., Hetzel, D.J.S. (1994). Characterization of 65 bovine microsatellites. Mamm. Genome 5, 84–90.
Murray, J.C., Buetow, K.H., Weber, J.L., Ludwigsen, S., Scherpbier-Heddema, T., Manion, F., Quillen, J., Sheffield, V.C., Sunden, S., Duyk, G.M., Weissenbach, J., Gyapay, G., Dib, C., Morrissette, J., Lathrop, G.M., Vignal, A., White, R., Matsunami, N., Gerken, S., Melis, R., Albertsen, H., Plaetke, R., Odelberg, S., Ward, D., Dausset, J., Cohen, D., Cann, H. (1994). A comprehensive human linkage map with centimorgan density. Science 265, 2049–2054.
Ostrander, E.A., Jong, P.M., Rine, J., Duyk, G. (1992). Construction of small-insert genomic DNA libraries highly enriched for microsatellite repeat sequences. Proc. Natl. Acad. Sci. USA 89, 3419–3423.
Skowronski, J., Plucienniczak, A., Beenarek, A., Jaworski, J. (1984). Bovine 1.709 satellite: recombination hotspots and dispersed repeated sequences. J. Mol. Biol. 177, 399–416.
Vaiman, D., Mercier, D., Moazami-Goudarzi, K., Eggen, A., Ciampolini, R., Lépingle, A., Velmala, R., Kaukinen, J., Varvio, S.L., Martin, P., Levéziel H, Guérin, G. (1994). A set of 99 cattle microsatellites: characterization, synteny mapping and polymorphism. Mamm. Genome 5, 288–297.
Weissenbach, J., Gyapay, G., Dib, C., Vignal, A., Morissette, J., Millasseau, P., Vaysseix, G., Lathrop, M. (1992). A second-generation linkage map of the human genome. Nature 359, 794–801.
Womack, J.E. (1987). Comparative gene mapping: a valuable tool for mammalian developmental studies. Dev. Genet. 8, 281–293.
Yap, E.P.H., McGee, J.O'D. (1991). Short PCR product yields improved by lower denaturation temperatures. Nucleic Acids Res. 19, 1713.
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Kirkpatrick, B.W., Bradshaw, M., Barendse, W. et al. Development of bovine microsatellite markers from a microsatellite-enriched library. Mammalian Genome 6, 526–528 (1995). https://doi.org/10.1007/BF00356169
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DOI: https://doi.org/10.1007/BF00356169