Abstract
A 2041 bp DNA fragment isolated from the Sxr (sex reversed) region of the mouse Y Chromosome (Chr) was sequenced and characterized. The sequence, pY8/b, contains four exons that are highly similar to 525 contiguous bases from the cDNA of human ubiquitin activating enzyme El. Two of the exons contain stop codons, indicating that pY8/b is not part of a functional gene. Sequences related to pY8/b were amplified from the Y Chr of the inbred mouse strain, C57BL/6J. These sequences may be portions of the recently discovered functional equivalent of pY8/b. Despite a high degree of similarity with the human El gene, the functional equivalent of pY8/b is not the mouse El gene, because unlike El, the functional equivalent of pY8/b is expressed in a tissue-specific manner. These data are discussed with respect to theory on the evolution of the mammalian Y Chr, and in particular, to the prediction that functional genes on the Y Chr have a male-specific function.
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Baron, B., Metezeau, P., Hatat, D., Roberts, C., Goldberg, M.E., and Bishop, C.E.: Cloning of DNA libraries from mouse Y chromosomes purified by flow cytometry. Som Cell Mol Gen 12: 289–295, 1986.
Bishop, C.E., Roberts, C., Michot J.-L., Nagamine, C., Winking, H., Guénet, J.-L., and Weith, A.: The use of specific DNA probes to analyse the Sxr mutation in the mouse. Development 101 (Suppl): 167–175, 1987.
Charlesworth, B.: The evolution of sex chromosomes. Science 251: 1030–1033, 1991.
Eicher, E.M. and Washburn, L.L.: Genetic control of primary sex determination in mice. Ann Rev Genet 20: 327–360, 1986.
Feinberg, A.P. and Vogelstein, B.: A technique for radiolabeling DNA restriction endonuclease fragments to a high specific activity. Anal Biochem 132: 6–13, 1983.
Feinberg, A.P. and Vogelstein, B.: A technique for radiolabeling DNA restriction endonuclease fragments to a high specific activity. Anal Biochem 132: 6–13, 1983.
Feinberg, A.P. and Vogelstein, B.: A technique for radiolabeling DNA restriction endonuclease fragments to a high specific activity. Anal Biochem 137: 266–267, 1984.
Fisher, E.M.C., Beer-Romero, P., Brown, L.G., Ridley, A., McNeil, J.A., Lawrence, J.B., Willard, H.F., Bieber, F.R., and Page, D.C.: Homologous ribosomal protein genes on the human X and Y chromosomes: Escape from X inactivation and possible implications for Turner syndrome. Cell 63: 1205–1218, 1990.
Gubbay, J., Collignon, J., Koopman, P., Capel, B., Economou, A., Munsterberg, A., Vivian, N., Goodfellow, P., and Lovell-Badge, R.: A gene mapping to the sex-determining region of the mouse Y chromosome is a member of a novel family of embryonically expressed genes. Nature 346: 245–250, 1990.
Hamuas, R.M., Brown, S.D.M., Kerr, J.T., Fisher, E.M., Romero, P., Vinn, A., and Page, D.: The mapping of the locus Rps4 to the X inactivation region in the mouse. Cytogenet Cell Genet, in press, 1992.
Handley, P.M., Mueckler, M., Siegel, N.R., Ciechanover, A., and Schwartz, A.L.: Molecular cloning, sequence, and tissue distribution of the human ubiquitin-activating enzyme El. Proc Natl Acad Sci USA 88: 258–262, 1991.
Koopman, P., Gubbay, J., Vivian, N., Goodfellow, P.N., and Lovell-Badge, R.: Male development of chromosomally female mice transgenic for Sry. Nature 351: 117–121, 1991.
Lau, Y.-F.C. and Chan, K.: The putative testis-determining factor and related genes are expressed as discrete-sized transcripts in adult gonadal and somatic tissues. Am J Hum Genet 45: 942–952, 1989.
Lawrence, C.B., Goldman, D.A., and Hood, R.A.: Optimized homology searches of the gene and protein sequence banks. Bull Math Biol 48: 569–583, 1986.
Lawrence, C.B. and Goldman, D.A.: Definition and identification of homology domains. CABIOS 4: 25–33, 1988.
Mardon, G. and Page, D.C.: The sex-determining region of the mouse Y chromosome encodes a protein with a highly acidic domain and 13 zinc fingers. Cell 56: 765–770, 1989.
Mardon, G., Mosher, R., Disteche, C.M., Nishioka, Y., McLaren, A., and Page, D.C.: Duplication, deletion and polymorphism in the sex-determining region of the mouse Y chromosome. Science 243: 78–80, 1989.
Mardon, G., Luoh, S.-W., Simpson, E.M., Gill, G., Brown, L.G., and Page, D.C.: Mouse Zfx protein is similar to Zfy-2: Each contains an acidic activating domain and 13 zinc fingers. Mol Cell Biol 10: 681–688, 1990.
McLaren, A., Simpson, E., Epplen, J.T., Studer, R., Koopman, P., Evans, E.P., and Burgoyne, P.S.: Location of the genes controlling H-Y antigen expression and testis determination on the mouse Y chromosome. Proc Natl Acad Sci USA 85: 6442–6445, 1988.
Mitchell, M.M., Simon, D., Affara, N., Ferguson-Smith, M., Avner, P., and Bishop, C.E.: Localization of murine X and autosomal sequences homologous to the human Y located testisdetermining region. Genetics 121: 803–809, 1989.
Mitchell, M.M., Woods, D.R., Tucker, P.K., Opp, J.S., and Bishop, C.E.: A candidate spermatogenic gene from the mouse Y chromosome may be a ubiquitin activating enzyme. Nature 354: 483–486, 1991.
Muller, H.J.: A gene for the fourth chromosome of Drosophila. J Exp Zool 17: 325–336, 1914.
Nagamine, C.M., Kaimin, C., Kozak, C.A., and Lau, Y.-F.C.: Chromosome mapping and expression of a putative testisdetermining gene in the mouse. Science 342: 80–83, 1989.
Nagamine, C.M., Chan, K., Hake, L.E., and Lau, Y.-F.C.: The two candidate testis-determining Y genes (Zfy-1 and Zfy-2) are differentially expressed in fetal and adult mouse tissues. Genes Develop 4: 63–74, 1990.
Palmer, M.S., Berta, P., Sinclair, A.H., Pym, B., and Goodfellow, P.N.: Comparison of human ZFY and ZFX transcripts. Proc Natl Acad Sci USA 87: 1681–1685, 1990.
Page, D.C., Mosher, R., Simpson, E.M., Fisher, E.M.C., Mardon, G., Pollack, J., McGillivray, B., de la Chapelle, A., and Brown, L.G.: The sex-determining region of the human Y chromosome encodes a finger protein. Cell 51: 1091–1104, 1987.
Reed, K.C. and Mann, D.A.: Rapid transfer of DNA from agarose gels to nylon membranes. Nucl Acids Res 13: 7207–7221, 1985.
Roberts, C., Weith, A., Passage, E., Michot, J.L., Mattei, M.G., and Bishop, C.E.: Molecular and cytogenetic evidence for the location of Tdy and Hya on the mouse Y chromosome short arm. Proc Natl Acad Sci USA 85: 6446–6449, 1988.
Saiki, R.K., Scharf, S., Faloona, F., Mullis, K.B., Horn, G.T., Erlich, A., and Arnheim, N.: Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230: 1350–1354, 1985.
Sanger, F., Nicklen, S., and Coulson, A.R.: DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467, 1977.
Schneider-Gadicke, A., Beer-Romero, P., Brown, L.G., Nussbaum, R., and Page, D.C.: ZFX has a gene structure similar to ZFY, the putative human sex determinant, and escapes X inactivation. Cell 75: 1247–1258, 1989.
Sinclair, A.H., Berta, P., Palmer, M.S., Hawkins, J.R., Griffiths, B.L., Smith, M.J., Foster, J.W., Frischauf, A.-M., Lovell-Badge, R., and Goodfellow, P.N.: A gene from the human sexdetermining region encodes a protein with homology to a conserved DNA-binding motif. Nature 346: 240–244, 1990.
Sinclair, A.H., Foster, J.W., Spencer, J.A., Page, D.C., Palmer, M., Goodfellow, P.N., and Graves, J.A.: Sequences homologous to ZFY, a candidate human sex-determining gene, are autosomal in marsupials. Nature 336: 780–783, 1988.
Wilkinson, K.D.: Purification and structural properties of ubiquitin. In M. Rechsteiner (ed.); Ubiquitin, pp. 5–38, Plenum Press, New York, 1988.
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Tucker, P.K., Phillips, K.S. & Lundrigan, B. A mouse Y Chromosome pseudogene is related to human ubiquitin activating enzyme E1 . Mammalian Genome 3, 28–35 (1992). https://doi.org/10.1007/BF00355838
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DOI: https://doi.org/10.1007/BF00355838