Abstract
Eighty sequences from the mouse genome database containing microsatellites (simple sequence repeats) have been analyzed for size variation among ten different inbred strains of mice; 62/80 (77.5%) showed polymorphism of at least three alleles. We have been able to detect all the polymorphims by agarose gel electrophoresis, often running the gels for up to 3 h. Between individual pairs of mouse strains to be used in chromosomal mapping studies in our laboratory, 35–60% polymorphism occurred. There are potentially enough microsatellites within the mouse and human genome to have a marker at every 1-cM distance. This simple approach will, therefore, continue to be useful in genome mapping studies, leading eventually to high-resolution maps of both the mouse and human genomes; this should allow for physical mapping and cloning of specific genes.
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Fowlis, G.A., Adelman, S., Knight, A.M. et al. PCR-analyzed microsatellites of the mouse genome—additional polymorphisms among ten inbred mouse strains. Mammalian Genome 3, 192–196 (1992). https://doi.org/10.1007/BF00355718
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DOI: https://doi.org/10.1007/BF00355718