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Points of rearrangements between plastid chromosomes: location of protein coding regions on broad bean chloroplast DNA

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Summary

By using genes from spinach and tobacco chloroplast DNA as probes we have localized 22 protein genes on a refined map of Vicia faba (broad bean) chloroplast DNA. The genes are rbcL, rpoA, psaA,B, psbA–E, atpA,B,E,FH,I, pet-A,B,D, and the ribosomal protein genes rp12, rps7, rps12, and rps19. In addition, we mapped sequences homologous to a conserved open reading frame previously found in an intron of a trnK gene. The results were used to map the relative positions of rearrangements that distinguish the broad bean and spinach plastid DNAs. By hybridization with small probes several regions on both DNAs with sequence homology to more than one chromosomal position were found. Intermediate segments are rearranged in broad bean relative to spinach. A spinach chloroplast DNA fragment that functions as an autonomously replicating sequence in yeast is shown to be missing in broad bean chloroplast DNA. This ars is located at a position in spinach DNA which has been a target for rearrangement in the broad bean chromosome. These results can be interpreted by assuming that the DNAs contain short repeat sequences at some conserved positions. We suggest a model for chloroplast DNA divergence assuming that short repeats exist which are the substrate for a recombination system. The few rearrangements between chloroplast chromosomes from a variety of plants may be explained as the result of selection against short repeats.

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Abbreviations

Kbp:

103 base-pairs

IR:

large inverted repeat unit

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Authors and Affiliations

  1. Department of Biochemistry, University of Arizona, 85721, Tucson, AZ, USA

    Christine Michalowski & Hans J. Bohnert

  2. Departments of Molecular and Cellular Biology, and Plant Sciences, University of Arizona, 85721, Tucson, AZ, USA

    Hans J. Bohnert

  3. Institut für Mikrobiologie der Universität, D-4000, Düsseldorf, Germany

    Karin D. Breunig

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  1. Christine Michalowski
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  2. Karin D. Breunig
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  3. Hans J. Bohnert
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Michalowski, C., Breunig, K.D. & Bohnert, H.J. Points of rearrangements between plastid chromosomes: location of protein coding regions on broad bean chloroplast DNA. Curr Genet 11, 265–274 (1987). https://doi.org/10.1007/BF00355400

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  • DOI: https://doi.org/10.1007/BF00355400

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