Summary
A full-length 1010-bp cDNA clone from Chlamydomonas moewusii coding for the precursor of a chlorophyll a/b-binding protein (CAB) was characterized. Northern analysis shows hybridization to a single 1150-base light-stimulated mRNA. Complementary hybrid-selected mRNAs were translated in vitro; SDS-PAGE indicates the synthesis of three polypeptides of 25, 27 and 28 kDa. Comparison of the deduced polypeptide sequence with other published CABs reveals greater similarity with PSII-associated proteins but, as with other algal CABs, our sequence does not meet established criteria for inclusion into either type I or type II, so branching of CABs into two types seems to have occurred after the divergence between algae and land plants.
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Communicated by B. B. Sears
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Larouche, L., Tremblay, C., Simard, C. et al. Characterization of a cDNA encoding a PSII-associated chlorophyll a/b-binding protein (CAB) from Chlamydomonas moewusii fitting into neither type I nor type II. Curr Genet 19, 285–288 (1991). https://doi.org/10.1007/BF00355056
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DOI: https://doi.org/10.1007/BF00355056