Summary
Experimental conditions for the separation of chromosomes from the model zygomycete Absidia glauca by rotating field electrophoresis were established. The sexually compatible strains of the mating type pair A. glauca CBS 100.48 (+) and CBS 101.48 (-) showed considerable differences in their electrophoretic karyotype. By Southern hybridization with homologous probes we have mapped the chromosomal locations for rDNA, the repetitive element rAg1, and the genes for actin and the elongation factor EF1. For the mapping of ubiquitin information we used a heterologous probe from U. maydis. The combination of electrophoretic karyotyping and Southern mapping proved to be a useful tool for characterizing mutant genotypes, which were induced by integrative transformation.
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References
Alvarez MI, Pelaez MI, Eslava AP (1980) Mol Gen Genet 179:447–452
Bergman K, Burke PV, Cerdá-Olmedo E, David CN, Delbrück M, Foster KW, Goodell EW, Heisenberg M, Meissner G, Zalokar M, Dennison DS, Shropshire W Jr (1969) Bacteriol Rev 33:99–157
Brody H, Carbon J (1989) Proc Natl Acad Sci USA 86:6260–6263
Burmester A, Wöstemeyer A, Wöstemeyer J (1990) Curr Genet 17:155–161
Carle GF, Olson MV (1984) Nucleic Acid Res 12:5647–5664
Cox EC, Vocke CD, Walter S, Gregg KY, Bain ES (1990) Proc Natl Acad Sci USA 87:8247–8251
Debets AJM, Holub EF, Swart K, van den Broek HWJ, Bos CJ (1990) Mol Gen Genet 224:264–268
Ellis JJ, Hesseltine CW (1965) Mycologia 57:222–235
Eslava AP, Alvarez MI, Lipson ED, Presti D, Kong K (1976) Mol Gen Genet 147:235–241
Feinberg A, Vogelstein B (1983) Anal Biochem 132:6–13
Gauger WL (1977) J Gen Microbiol 101:211–217
Howlett BJ (1989) Exp Mycol 13:199–202
Lasker BA, Carle GF, Kobayashi GS, Medoff G (1989) Nucleic Acids Res 17:3783–3793
Masel A, Braithwaite K, Irwin J, Manners J (1990) Curr Genet 18:81–86
McCluskey K, Mills D (1990) Molecular Plant-Microbe Interactions 3:366–373
Mesland DAM, Huisman JG, van den Ende H (1974) J Gen Microbiol 80:111–117
Osiewacz HD, Clairmont A, Huth M (1990) Curr Genet 18:481–483
Petes TD (1979) Proc Natl Acad Sci USA 76:410–414
Rollo F, Ferracuti T, Pacilli A (1989) Curr Genet 16:477–479
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Schwarz DC, Cantor RC (1984) Cell 34:67–75
Smith CL, Matsumoto T, Niwa O, Kleo S, Fan J-B, Yanagida M, Cantor CR (1987) Nucleic Acids Res 15:4481–4489
Southern EM (1975) J Mol Biol 98:503–517
Southern EM, Anand R, Brown WRA, Fletcher DS (1987) Nucleic Acids Res 15:5925–5943
Werkman BA (1976) Arch Microbiol 109:209–213
Wöstemeyer J (1985) Eur J Biochem 146:443–448
Wöstemeyer J, Burmester A (1986) Curr Genet 10:903–907
Wöstemeyer J, Brockhausen-Rohdemann E (1987) Curr Genet 12:435–441
Wöstemeyer A, Teepe H, Wöstemeyer J (1990) Curr Genet 17:163–168
Ziegler A, Geiger KH, Ragoussis J, Szalay G (1987) J Clin Chem Clin Biochem 25:578–579
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Kayser, T., Wöstemeyer, J. Electrophoretic karyotype of the zygomycete Absidia glauca: evidence for differences between mating types. Curr Genet 19, 279–284 (1991). https://doi.org/10.1007/BF00355055
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DOI: https://doi.org/10.1007/BF00355055