Abstract
In order to acquire more in-depth information on the site(s) of hemopoiesis in marine animals, we successfully cultured pharyngeal explants of the tunicate Styela clava over a period of 82 d. Transmission electron microscopy of resident hemocytes within explants revealed changes in hemocyte composition. Hemoblast-like cells increased shortly after commencement of the cultures (8.1% at Day 0 increasing to a maximum of 28.7% at Day 7). Autoradiography using 3H-thymidine incorporation confirmed that hemocyte proliferation in pharyngeal explants still continued after 37 d culture. During culture, the migration of many free cells into the medium resulted in sparse, residential hemocytes in the pharyngeal explants. Hemocyte migration increased by up to 4.3x105 cells/explant (max.) at 17 to 24 d, but finally decreased to 4.9x104 cells/explant at 75 to 82 d. Vital neutral-red staining revealed that many emerging cells were not hemocytes such as those found in the normal hemolymph. The continued development of in vitro approaches will strengthen analyses of immune-defense responses in tunicates which, as protochordates, are the immediate invertebrate ancestors of vertebrates.
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Communicated by M. G. Hadfield, Honolulu
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Sawada, T., Zhang, J. & Cooper, E.L. Sustained viability and profileration of hemocytes from the cultured pharynx of Styela clava . Marine Biology 119, 597–603 (1994). https://doi.org/10.1007/BF00354323
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DOI: https://doi.org/10.1007/BF00354323