Abstract
Mouse/hamster somatic cell hybrids form a valuable resource for mouse gene mapping. Characterization of these hybrids by isozyme analysis can be technically demanding and time-consuming. Species-specific polymerase chain reaction (PCR), where a mouse gene but not its homolog in the hamster is amplified, can provide an alternative means of characterization. Mouse-specific primers have been designed for at least one gene on each of the mouse autosomes and the X Chromosome (Chr). Primers are chosen to correspond to untranslated regions of the mouse gene concerned, in order to decrease the chance of crosshybridization with the homologous hamster gene. These primer sequences are presented, together with the conditions for their use.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abbott, C., and Povey, S.: Development of human chromosome-specific PCR primers for characterization of somatic cell hybrids. Genomics 9: 73–77, 1991.
Avner, P., Amar, L., Dandolo, L. and Guénet, J.-L.: Genetic analysis of the mouse using interspecific crosses. Trends Genet 4: 18–23, 1988.
Brunet, J., Dosseto, M., Denizot, F., Mattei, M-G., Clark, W., Haqqi, T., Ferrier, P., Nabholz, M., Schmitt-Verhulst, A-M., Luciani, M-F., and Golstein, P.: The inducible cytotoxic T-lymphocyte-associated gene transcript CTLA-1 sequence and gene localization to mouse chromosome 14. Nature 322: 268–271, 1986.
Chen, M., Shimada, T., Moulton, A., Cline, A., Humphries, R., Maizel, J. and Nienhuis, A.: The functional human dihydrofolate reductase gene. J Biol Chem 259: 3933–3943, 1984.
Dush, M., Sikela, J., Khan, S., Tischfield, J. and Stambrook, P.: Nucleotide sequence and organization of the mouse Aprt gene: Presence of a coding region common to animal and bacterial phosphoribosyl-transferases that has a variable intron/exon arrangement. Proc Natl Acad Sci USA 82: 2731–2735, 1985.
Eisenberg, L. and Jänne, O.: Nucleotide sequence of the 5′ flanking region of the murine ornithine decarboxylase gene. Nucl Acids Res 17: 2359, 1989.
Grayson, D., Costa, R., Xanthopoulos, K. and Darnell, J.: A cell-specific enhancer of the mouse Aat gene has multiple functional regions and corresponding protein-binding sites. Mol Cell Biol 8: 1055–1066, 1988.
Hochgeschwender, U., Sutcliffe, J.A. and Brennan, M.B.: Construction and screening of a genomic library specific for mouse chromosome 16. Proc Natl Acad Sci USA 86: 8482–8486, 1989.
Hurt, M., Chodchoy, N. and Marzluff, W.: The mouse histone H2a.2 gene from chromosome 3. Nucl Acids Res 17: 8876, 1989.
Kudo, A., Sakaguchi, N. and Melchers, F.: Organization of the murine Ig-related λ5 gene transcribed selectively in pre-B lymphocytes. EMBO J 6: 103–107, 1987.
McDonald J., Lin, F. and Goldwasser, E.: Cloning, sequencing and evolutionary analysis of the mouse erythropoietin gene. Mol Cell Biol 6: 842–848, 1986.
Melton, D., Konecki, D., Brennand, J. and Caskey, T.: Structure, expression, and mutation of the Hprt gene. Proc Natl Acad Sci USA 81: 2147–2151, 1984.
Müller, G., Ruppert, S., Schmid, E. and Schültz, G.: Functional analysis of alternatively spliced tyrosinase gene transcripts. EMBO J 7: 2723–2730, 1988.
Panthier, J., Dreyfus, M., Tronik-LeRoux, D. and Rougeon, F.: Mouse kidney and submaxillary gland renin genes differ in their 5′ putative regulatory sequences. Proc Natl Acad Sci USA 81: 5489–5493, 1984.
Pilz, A., Fox, M., Povey, S. and Abbott, C.: Abstract: Construction of mouse/hamster somatic cell hybrids with translocation breakpoints on MMU2 and their characterization by PCR. Mouse Genome, in press.
Schimenti, J., Cebra-Thomas, J., Decker, C., Islam, S., Pilder, S. and Silver, L.M.: A candidate gene family for the mouse Tcr locus responsible for haploid effects on sperm function. Cell 55: 71–78, 1988.
Schughart, K., Pravtcheva, D., Newman, M., Hunihan, L., Jiang, Z. and Ruddle, F.: Isolation and regional localization of the murine homeobox-containing gene Hox 3.3 to mouse chromosome region 15E. Genomics 5: 76–83, 1989.
Shi, Y., Rodriguez, M. Shahan, K. and Derman, E.: Subfamily of submaxillary gland-specific Mup genes: Chromosomal linkage and sequence comparison with liver-specific Mup genes. Nucl Acids Res 17: 6191–6203, 1989.
Sobieszczuk, P., Gonda, T., and Dunn, A.: Structure and biological activity of the transcriptional initiation sequences of the murine c-myb oncogene. Nucl Acids Res 17: 9593–9611, 1989.
Wang, J., Ledley, F., Goff, S., Lee, R., Groner, Y. and Baltimore, D.: The mouse c-abl locus: Molecular cloning and characterization. Cell 36: 349–356, 1984.
Wiginton, D., Kaplan, D., States, J., Adeson, A., Perme, C., Bilyk, I., Vaughn, A., Lattier, J. and Hutton, J.: Complete sequence and structure of the gene for human adenosime deaminase. Biochemistry 25: 8234–8244, 1986.
Williams, S., Bruckheimer, S., Lusis, A., LeBoeuf, R. and Kinniburgh, A.: Mouse apolipoprotein A-IV gene: Nucleotide sequence and induction by a high lipid diet. Mol Cell Biol 6: 3807–3814, 1986.
Yachou, A., Renaudie, F., Grandchamp, B. and Beaumont, C.: Nucleotide sequence of the mouse Ferritin H chain gene. Nucl Acids Res 17: 8005, 1989.
Yeung, C., Ingolia, D., Roth, D., Shoemaker, C., Al-Ubaidi, M., Yen, J., Ching, C., Bobonis, C., Kaufman, R. and Kellems, R.: Identification of functional murine ADA cDNA clones by complementation in E. coli. J Biol Chem 260: 299–307, 1985.
Zakut-Houri, R., Oren, M., Bienz, B., Lavie, V., Hazum, S. and Givol D.: A single gene and a pseudogene for the cellular tumour antigen P53. Nature 306: 594, 1983.
Zhu, L. and Jones, P.: Complete sequence of the murine invariant chain (Ii) gene. Nucl Acids Res 17: 447–448, 1989.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Abbott, C. Characterization of mouse-hamster somatic cell hybrids by PCR: A panel of mouse-specific primers for each chromosome. Mammalian Genome 2, 106–109 (1992). https://doi.org/10.1007/BF00353858
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00353858