Abstract
A cDNA library from mink spleen was constructed by use of the phage λgt11. The library was screened using polyvalent serum raised against the mink immunoglobulin λ chain. As a result, several clones expressing mink immunoglobulin λ light chains were identified. Sequencing of one of the clones with an 803 bp insert was performed. The insert comprised nearly the entire coding region for the mature λ light immunoglobulin gene with the exception of the leader polypeptide and several amino acids of the RF1 region of the V segment. Compared with the rabbit, mouse and human λ light immunoglobulin genes, the homology of the cloned sequence was found to be highest relative to the rabbit gene. With the cloned mink cDNA containing the C-region only as a probe, the DNAs from mink-Chinese hamster hybrid clones were studied. The results of segregation analysis of this mink cDNA sequence and mink chromosomes in the mink-Chinese hamster clone panel allowed us to assign the gene for the λ light immunoglobulin constant polypeptide (IGLC) to mink Chromosome (Chr) 4.
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Khlebodarova, T.M., Matveeva, N.M., Serov, O.L. et al. The mink gene for the λ light immunoglobulin chain: Characterization of cDNA and chromosomal localization. Mammalian Genome 2, 96–99 (1992). https://doi.org/10.1007/BF00353856
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DOI: https://doi.org/10.1007/BF00353856