Skip to main content
SpringerLink
Log in

A new method for heterokaryon formation in Phycomyces

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

An efficient method is described for obtaining heterokaryons in Phycomyces by grafting stage I sporangiophores of two strains to one another. The grafts are successful in over 70% of the cases and success may be ascertained after 10 hours. The successful grafts in almost all cases regenerate one or more sporangiophores and sporangia, and about 85% of the regenerates are heterokaryotic.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Burgeff, H.: Untersuchungen über Variabilität, Sexualität, und Erblichkeit bei Phycomyces nitens Kunze. I. Flora 107, 259–316 (1914).

    Google Scholar 

  • Cerdá-Olmedo, E., Reau, P.: Genetic classification of the lethal effects of various agents on heterokaryotic spores of Phycomyces. Mutation Res. 9, 369–384 (1970).

    Google Scholar 

  • De La Guardia, M.D., Aragón, C.M.G., Murillo, F.J., Cerdá-Olmedo, E.: A carotenogenic enzyme aggregate in Phycomyces: Evidence from quantitative complementation. Proc. nat. Acad. Sci. (Wash.) 68, 2012–2015 (1971).

    Google Scholar 

  • Demerec, M., Adelberg, E.A., Clark, A.J., Hartman, P.E.: A proposal for a uniform nomenclature in bacterial genetics. Genetics 54, 61–76 (1966).

    Google Scholar 

  • Gruen, H.E.: Growth and development of isolated Phycomyces sporangiophores. Plant Physiol. 34, 158–168 (1959).

    Google Scholar 

  • Gruen, H.E., Ootaki, T.: Regeneration on Phycomyces sporangiophores. I. Induction by decapitation and basal cutting on sporangiophores attached to mycelium and after isolation. Canad. J. Bot. 48, 55–65 (1970).

    Google Scholar 

  • Gruen, H.E., Ootaki, T.: Regeneration on Phycomyces sporangiophores. III. Grafting of sporangiophore segments, weight changes, and protoplasmic streaming in relation to regeneration. Canad. J. Bot. 50, 139–158 (1972).

    Google Scholar 

  • Heisenberg, M., Cerdá-Olmedo, E.: Segregation of heterokaryons in the asexual cycle of Phycomyces. Molec. gen. Genet. 102, 187–195 (1968).

    Google Scholar 

  • Meissner, G., Delbrück, M.: Carotenes and retinal in Phycomyces mutants. Plant Physiol. 43, 1279–1283 (1968).

    Google Scholar 

  • Ootaki, T., Lighty, A. Crafts, Delbrück, M., Hsu, W.-J.: Complementation between mutants of Phycomyces deficient with respect to carotenogenesis. Molec. gen. Genet. 121, 57–70 (1973).

    Google Scholar 

  • Villet, R.H.: Genetic curing of blindness in Phycomyces blakesleeanus: a quantiative assessment of dominance. Nature (Lond.) 225, 453–454 (1970).

    Google Scholar 

  • Villet, R.H.: Microinjection of Phycomyces. Selection of a strain for possible biological assay of photoreceptor pigment. Plant Physiol. 49, 273–274 (1972).

    Google Scholar 

  • Weide, A.: Beobachtungen an Plasmaexplantaten von Phycomyces. Arch. exp. Zellforsch. 23, 299–337 (1939).

    Google Scholar 

  • Zalokar, M.: Intracellular centrifugal separation of organelles in Phycomyces. J. Cell Biol. 41, 494–509 (1969).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Biology, California Institute of Technology, Pasadena, California, USA

    T. Ootaki

Authors
  1. T. Ootaki
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by G. Melchers

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ootaki, T. A new method for heterokaryon formation in Phycomyces . Molec. gen. Genet. 121, 49–56 (1973). https://doi.org/10.1007/BF00353692

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00353692

Keywords

Search

Navigation