Abstract
Gene mapping in cattle has progressed rapidly in recent years largely owing to the introduction of powerful genetic markers, such as the microsatellites, and through advances in physical mapping techniques such as synteny mapping and fluorescence in situ hybridization (FISH). Microsatellite markers are often not physically mapped because they are generally isolated from small insert plasmid libraries, which makes their chromosomal localization inefficient. In this report we describe the FISH mapping of a large group of cosmid-derived bovine microsatellite markers, as our contribution to the European mapping initiative, BovMap. One objective of BovMap is to develop a set of anchored loci for the cattle genome map.
Two cosmid libraries were screened with probes corresponding to the (AC) n microsatellite motif. Positive clones were mapped by FISH, and then a subset was further analyzed by sequencing the region flanking the microsatellite repeat. In total, 58 clones were hybridized with chromosomes and identified loci on 22 of the 31 different bovine chromosomes. Three clones contained satellite DNA. Two or more markers were placed on 12 chromosomes. Sequencing of the microsatellites and flanking regions was performed directly from 43 cosmids, as previously reported (Ferretti et al. Anim. Genet. 25, 209–214, 1994). Primers were developed for 39 markers and used to describe the polymorphism associated with the corresponding loci.
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Barendse, W., Armitage, S.M., Ryan, A.M., Moore, S.S., Clayton, D., Georges, M., Womack, J.E., Hetzel, D.J.S. (1993). A genetic map of DNA markers on bovine chromosome 1. Genomics 18, 602–608.
Barendse, W., Armitage, S.M., Kossarek, L.M., Shalom, A., Kirkpatrick, B.W., Ryan, A.M., Clayton, D., Li, L., Neiberg, H.L., Zhang, N., Grosse, W.M., Weiss, J., Creighton, P., McCarthy, F., Ron, M., Teale, A.J., Fries, R., McGraw, R.A., Moore, S.S., Geoerges, M., Soller, M., Womack, J.E., Hetzel, D.J.S. (1994) A genetic linkage map of the bovine genome. Nature Genet. 6, 227–235.
Bishop, M.D., Kappes, S.M., Keele, J.W., Stone, R.T., Sunden, S.L.F., Hawkins, G.A., Solinas Toldo, S., Fries, R., Grosz, M.D., Yoo, J., Beattie, C.W. (1994). A genetic linkage map for cattle. Genetics 136, 619–639.
Dietrich, W.F., Miller, J.C., Steen, R.G., Merchant, M., Damron, D., Nahf, R., Gross, A., Joyce, D.C., Wessel, M., Dredge, R.D., Marquis, A., Stein, L.D., Goodman, N., Page, D.C., Lander, E.S. (1994). A genetic map of the mouse with 4,006 simple sequence length polymorphisms. Nature Genet 7, 220–245.
Eggen, A., Bahri-Darwich, I., Mercier, D., Vaiman, D., Cribiu, E.P. (1994). Assignment of bovine synteny group U2 to chromosome 9. Anim. Genet. 25, 183–185
Ellegren, H., Johansson, M., Chowdhary, B., Marklund, S., Ruyter, D., Marklund, L., Bräuner-Nielsen, P., Edfors-Lilja, I., Gustavsson, I., Juneja, R.K., Andersson, L. (1993). Assignment of 20 microsatellite markers to the porcine linkage map. Genomics 16, 431–439.
Ellegren, H., Chowdhary, B., Johansson, M., Andersson, L. (1994a). Integrating the porcine physical and linkage map using cosmid-derived markers. Anim. Genet. 25, 155–164.
Evans, G.A., Lewis, K., Rothenber, B.E. (1989). High efficiency vectors for cosmid microcloning and genome analysis. Gene 79, 9–20.
Ferretti, L., Leone, P., Pilla, F., Zhang, Y., Nocart, M., Guérin, G. (1994). Direct characterization of bovine microsatellites from cosmids: polymorphism and synteny mapping. Anim. Genet. 25, 209–214.
Fries, R. (1993). Mapping the bovine genome: methodological aspects and strategy. Anim. Genet. 24, 111–116.
Fries, R., Eggen, A., Womack, J.E. (1993). A bovine genome map. Mamm. Genome 4, 405–428.
Gallagher D.S., Ryan, A.M., Liou, L.S., Sastry, K.N., Womack, J.E. (1993). Somatic cell mapping of conglutinin (CGN1) to cattle syntenic group U29 and fluorescence in situ localization to Chromosome 28. Mamm. Genome 4, 716–719.
Gyapay, G., Morissette, J., Vignal, A., Dib, C., Fizames, C., Millasseau, P., Marc S, Bernardi G., Lathrop, M., Weissenbach, J. (1994). The 1993–94 Généthon human genetic linkage map. Nature Genet. 7, 246–339.
Hearne, C.M., Ghosh, S., Todd, J.A. (1992). Microsatellites for linkage analysis of genetic traits. Trends Genet. 8, 288–294.
ISCNDA (1989) International system for cytogenetic nomenclature of domestic animals. Cytogenet. Cell Genet. 53, 65–79.
Leveziel, H. (1995). Development of genetic and physical marker maps of the bovine genome (Bovmap project). In Biotechnology (1992–1994) Progress Report, P. de Taxis du Pöet, A. Vassarotti, J. Baselga de Elorzs, eds. (Brussels: European Commission), pp. 294–300.
Lichter, P., Tang, C.-J., Call, K., Hermanson, G., Evans, G.A., Housman, D., Ward, D.C., (1990). High resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. Science 247, 64–69.
Mezzelani, A., Solinas Toldo, S., Nocart, M., Guerin, G., Ferretti, F., Fries, R. (1994) Mapping of syntenic groups U7 and U27 to bovine chromosomes 25 and 12, respectively. Mamm. Genome 5, 574–576.
Moore, S.S., Byrne, K., Berger, K.T., Barendse, W., McCarthy, F., Womack, J.E., Hetzel, D.J.S. (1994). Characterization of 65 bovine microsatellites. Mamm. Genome 5, 84–90.
Ried, T., Baldini, A., Rand, T.C., Ward, D.C. (1992) Simultaneous visualization of seven different probes by in situ hybridization using combinatorial fluorescence and digital imaging microscopy. Proc. Natl. Acad. Sci. USA 89, 1388–1392.
Solinas Toldo, S., Fries, R., Steffen, P., Neibergs, H.L., Barendse, W., Womack, J.E., Hetzel, D.J.S., Stranzinger, G. (1993). Physically mapped, cosmid-derived microsatellite markers as anchor loci on bovine chromosomes. Mamm. Genome 4, 720–727.
Solinas Toldo, S., Mezzelani, A., Hawkins, G.A., Bishop, M., Olsaker, I., Mackinlay, A.G., Ferretti, L., Fries, R., (1995). Identification of bovine chromosomes 1 to 7 based on fluorescence in situ hybridization. Cytogenet. Cell Genet. 69, 1–6.
Steffen, P.J. (1992). Systematische Suche nach hochpolymorphen Markeloci im Rindergenom. Dissertation, ETH Zurich, No. 9852.
Vaiman, D., Mercier, D., Moazami-Goudarzi, K., Eggen, A., Ciampolini, R., Léplingle, A., Velmala, R., Kaukinen, J., Varvio, S.L., Martin, P., Levéziel, H., Guérin, G. (1994). A set of 99 cattle microsatellites: characterization, synteny mapping, and polymorphism. Mamm. Genome 5, 288–297.
Weber, J.L. (1990). Informativeness of human (dC-dA)n · (dG-dT)n polymorphisms. Genomics 7, 524–530.
Yuille, M.A.R., Goudie, D.R., Affara, N.A., Ferguson-Smith, M.A. (1991). Rapid determination of sequence flanking microsatellites. Nucleic Acids Res. 19, 1950.
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Mezzelani, A., Zhang, Y., Redaelli, L. et al. Chromosomal localization and molecular characterization of 53 cosmid-derived bovine microsatellites. Mammalian Genome 6, 629–635 (1995). https://doi.org/10.1007/BF00352370
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DOI: https://doi.org/10.1007/BF00352370