Abstract
Microsatellite sequences, such as dinucleotide repeats, show a high degree of polymorphism in eukaryotic DNA. These sequences are convenient as genetic markers and can be analyzed by the polymerase chain reaction (PCR). We have assessed the frequency of length variants in 18 mononucleotide repeats in mouse DNA and find that the variability is similar to that reported for dinucleotide repeats. Nine of the 18 repeat sequences (50%) have three or more alleles in the strains tested. Ten of these repeat sequences have been mapped using strain distribution patterns (SDPs) in recombinant inbred (RI) strains.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ceci, J.D., Kingsley, D.M., Silan, C.M., Copeland, N.G., and Jenkins, N.A.: An interspecific backcross linkage map of the proximal half of mouse Chromosome 14. Genomics 6: 673–678, 1990.
Czosnek, H.H., Bienz, B., Givol, D., Zakut-Houri, R., Pravtcheva, D.D., Ruddle, F.H., and Oren, M.: The gene and the pseudogene for mouse p53 cellular tumor antigen are located on different chromosomes. Mol CelL Biol 4: 1638–1640, 1984.
Devereux, J., Haeberli, P., and Smithies, O.: A comprehensive set of sequence analysis programs for the VAX. Nucl Acids Res 12: 387–395, 1984.
Economou, E.P., Bergen, A.W., Warren, A.C., and Antonarakis, S.E.: The polydeoxyadenylate tract of the Alu repetitive element is polymorphic in the human genome. Proc Natl Acad Sci USA 87: 2951–2954, 1990.
Flavell, R.A., Van den Berg, F.M., and Grosveld, G.C.: Isolation and characterization of the oligo (dA-dT) clusters and their flanking DNA segments in the rabbit genome. J Mol Biol 115: 715–741, 1977.
Frankel, W.N., Stoye, J.P., Taylor, B.A., and Coffin, J.M.: Genetic identification of enogenous polytropic proviruses by using recombinant inbred mice. J Virol 63: 3810–3821, 1989.
Gebhard, W., Meitinger, T., Hochtl, J., and Zachau, H.G.: A new family of interspersed repetitive DNA sequences in the mouse genome. J Mol Biol 157: 453–471, 1972.
Hamada, H., Petrino, M.G., and Kakunaga, T.: A novel repeat element with Z-DNA-forming potential is widely found in evolutionarily diverse eukaryotic genomes. Proc Natl Acad Sci USA 79: 6465–6469, 1982.
Hastie, N.: Highly repeated DNA families in the genome of Mus musculus. In M.F. Lyon and A.G. Searle (eds), Genetic Variants and Strains of the Laboratory Mouse, pp. 559–573, Oxford University Press, Oxford, 1989
Hillyard, A.L., Doolittle, D.P., Davisson, M.T., and Roderick, T.H.: Locus map of mouse with comparative map points of human on mouse. The Jackson Laboratory, Bar Harbor, Maine, December 1990.
Litt, M. and Luty, J.A.: A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. Am J Hum Genet 44: 397–401, 1989.
Love, J.M., Knight, A.M., McAleer, M.A., and Todd, J.A.: Towards construction of a high resolution map of the mouse genome using PCR-analyzed microsatellites. Nucl Acids Res 18: 4123–4130, 1990.
Neumann, P.E.: Two-locus linkage analysis using recombinan inbred strains and Bayes' theorem. Genetics 126: 277–284, 1990.
Orita, M., Sekiya, T., and Hayashi, K.: DNA sequence polymorphisms in Alu repeats. Genomic 8: 271–278, 1990.
Shenkin, A. and Burdon, R.H.: Deoxyadenylate-rich and deoxyguanylate-rich regions in mammalian DNA. J Mol Biol 85: 19–39, 1974.
Tautz, D.: Hypervariability of simple sequences as a general source for polymorphic DNA markers. Nucl Acids Res 17: 6463–6471, 1989.
Tautz, D. and Renz, M.: Simple sequences are ubiquitous repetitive components of eukaryotic genomes. Nucl Acids Res 12: 4127–4138, 1984.
Weber, J.L.: Informativeness of human (dC-dA)n.(dG-dT)n polymorphisms. Genomics 7: 524–530, 1990.
Weber, J.L. and May, P.E.: Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. Am J Hum Genet 44: 388–396, 1989.
Zuliani, G. and Hobbs, H.H.: A high frequency of length polymorphisms in repeated sequences adjacent to Alu sequences. Am J Hum Genet 46: 963–969, 1990.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Aitman, T.J., Hearne, C.M., McAleer, M.A. et al. Mononucleotide repeats are an abundant source of length variants in mouse genomic DNA. Mammalian Genome 1, 206–210 (1991). https://doi.org/10.1007/BF00352326
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00352326