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Replication banding studies in two cyprinid fishes

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Abstract

The use of the in vivo 5-bromodeoxyuridine (BrdU) incorporation technique made it possible to induce highly reproducible replication bands of the RBA type in two species of the order Cypriniformes (Teleostei), Rutilus rutilus and Scardinius erythrophthalmus. This allowed unequivocal identification of each individual chromosome by its banding pattern and the preparation of species specific karyotypes. Comparison of RBA-banded karyotypes of these two closely related fish species revealed that the majority of the chromosomes could be homoeologized either directly, or by assuming paracentric or pericentric inversions.

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References

  • Almeida Toledo, LF, Viegas-Pequinot, E, Foresti, F, Toledo Filho, SA, Dutrillaux, B (1988) BrdU replication patterns demonstrating chromosome homoeologies in two fish species of the genus Eigenmannia. Cytogenet Cell Genet 48:117–120

    Google Scholar 

  • Amemiya, CT, Gold, JR (1988) Chromosomal NORs as taxonomic and systematic characters in North American cyprinid fishes. Genetica 76:81–90

    Google Scholar 

  • Bernardi, G (1989) The isochore organization of the human genome. Annu Rev Genet 23:637–661

    Google Scholar 

  • Bernardi, G, Olofsson, B, Filipski, J, Zerial, M, Salinas, J, Cuny, G, Meunier-Rotival, M, Rodier, F (1985) The mosaic genome of warm blooded vertebrates. Science 228:953–958

    Google Scholar 

  • Casperson, T, Zech, L, Johansson, C, Modest, EJ (1970) Identification of human chromosomes by DNA-binding fluorescent agents. Chromosoma 30:215–227

    Google Scholar 

  • Cau, A, Salvadori, S, Deiana, AM, Bella, JL, Mezzanotte, R (1988) The characterisation of Muraena helena L. mitotic chromosomes: karyotype C-Banding, NOR and in situ digestion with endonucleases. Cytogenet Cell Genet 47:223–226

    Google Scholar 

  • Comings, DE (1978) Mechanisms of chromosome banding and implications for chromosome structure. Annu Rev Genet 12:25–46

    Google Scholar 

  • Corneo, G, Ginelli, E, Soave, C, Bernardi, G (1968) Isolation and characterization of mouse and guinea pig satellite DNAs. Biochemistry 7:4373–4379

    Google Scholar 

  • Cuny, G, Soriano, P, Macaya, G, Bernardi, G (1981) The major components of the mouse and human genomes. 1. Preparation, basic properties, and compositional heterogeneity. Eur J Biochem 115:227–233

    Google Scholar 

  • Delany, ME, Bloom, SE (1984) Replication banding patterns in the chromosomes of the rainbow trout. J Hered 75:431–434

    Google Scholar 

  • Dutrillaux, B, Laurent, C, Couturier, J, Lejeune, L (1973) Coloration par l'acridine orange de chromosomes préalablement traités par le 5-bromodéoxyuridine (BUdR). CR Acad Sci III 276:3179–3181

    Google Scholar 

  • Filipski, J, Thiery, JP, Bernardi, G (1973) An analysis of the bovine genome by Cs2SO4/Ag+ density gradient centrifugation. J Mol Biol 80:177–197

    Google Scholar 

  • Foresti, F, Almeida Toledo, LF, Toledo, SA (1981) Polymorphic nature of nucleolus organizer regions in fishes. Cytogenet Cell Genet 31:137–144

    Google Scholar 

  • Galetti, PMJr, Foresti, F (1986) Evolution of the ZZ/ZW system in Leporinus (Pisces, Anastomidae). Cytogenet Cell Genet 43:43–46

    Google Scholar 

  • Giles, V, Thode, G, Alvarez, MC (1988) Early replication bands in two Scorpion fishes, Scorpaena porcus and S. notata (Order Scorpaneiformes). Cytogenet Cell Genet 47:80–83

    Google Scholar 

  • Goldman, MA, Holmquist, GP, Gray, MC, Caston, LA, Nay, A (1984) Replication timing of genes and middle repetitive sequences. Science 224:686–692

    Google Scholar 

  • Goodpasture, C, Bloom, SE (1975) Visualization of nucleolar organizer regions III. Mammalian chromosomes using silver staining. Chromosoma 53:37–50

    Google Scholar 

  • Holmquist, G (1988) DNA sequences of G-bands and R-bands. In: Adolph, KW (ed) Chromosome and chromatin structure, vol II. CRC Press, Boca Raton

    Google Scholar 

  • Holmquist, G, Gray, M, Porter, T, Jordan, J (1982) Characterisation of Giemsa dark- and light-band DNA. Cell 31:121–129

    Google Scholar 

  • Hudson, AP, Cuny, G, Cortadas, J, Haschemeyer, AEV, Bernardi, G (1980) An analysis of fish genomes by density gradient centrifugation. Eur J Biochem 112:203–210

    Google Scholar 

  • ISCN (1985) An international system for human cytogenetic nomenclature, Harnden, DG, Klinger, HP (eds) Published in collaboration with Cytogenet Cell Genet, Karger, Basel 1985; also in Birth Defects. (Original article series, vol 21, no 1.) March of Dimes Birth Defects Foundation, New York 1985

    Google Scholar 

  • Kligerman, AD, Bloom, SE (1977) Rapid chromosome preparations from solid tissues of fishes. J Fish Res Board Can 34:266–269

    Google Scholar 

  • Latt SA, Munroe SH, Disteche C, Rogers WE, Cassel DM (1977) Uses of fluorescent dyes to study chromosome structure and replication. In: Dela Chapelle A, Sorsa M (eds) Chromosomes today 6. Oxford Chromosome Conference, Edinburgh, pp 27–36

  • Macaya, G, Thiery, JP, Bernardi, G (1976) An approach to the organisation of eukaroytic genomes at a macromolecular level. J Mol Biol 108:237–254

    Google Scholar 

  • Mayr, B, Rab, P, Kalat, M (1986) NORs and counterstain-enhanced fluorescence studies in Cyprinidae of different ploidy level. Genetica 69:111–118

    Google Scholar 

  • Mayr, B, Kalat, M, Rab, P, Lambrou, M (1987) Band karyotypes and specific types of heterochromatins in several species of European percid fishes (Percidea, Pisces). Genetica 75:199–205

    Google Scholar 

  • Medrano, L, Bernardi, G, Couturier, J, Dutrillaux, B, Bernardi, G (1988) Chromosome banding and genome compartmentalization in fishes. Chromosoma 96:178–183

    Google Scholar 

  • Pizon, V, Cuny, G, Bernardi, G (1984) Nucleotide sequence organisation in the very small genome of a tetradontid fish, Arothron diadematus. Eur J Biochem 140:25–30

    Google Scholar 

  • Post, A (1965) Vergleichende Untersuchungen der Chromosomenzahlen bei Süßwasser-Teleosteern. Z Zool Syst Evol 3:47–93

    Google Scholar 

  • Schempp, W, Schmid, M (1981) Chromosome banding in amphibia. VI. BrdU-replication patterns in Anura and demonstration of XX/XY sex chromosomes in Rana esculenta. Chromosoma 83:697–710

    Google Scholar 

  • Schmid, M (1978) Chromosome banding in Amphibia. Chromosoma 68:131–148

    Google Scholar 

  • Schmid, M, Guttenbach, M (1988) Evolutionary diversity of reverse (R) fluorescent chromosome bands in vertebrates. Chromosoma 97:101–114

    Google Scholar 

  • Schnedl, W (1971) Banding pattern of human chromosomes. Nature New Biol 233:93–94

    Google Scholar 

  • Schweizer, D (1976) Reverse fluorescent chromosome banding with chromomycin and DAPI. Chromosoma 58:307–324

    Google Scholar 

  • Schweizer, D, Ambros, P, Andrle, M (1978) Modification of DAPI banding on human chromosomes by prestaining with a DNA-binding oligopeptide antibiotic, distamycin A. Exp Cell Res 111:327–332

    Google Scholar 

  • Schweizer, D, Loidl, J, Hamilton, B (1987) Heterochromatin and the phenomenon of chromosome banding. In: Hennig, W (ed) Results and problems in cell differentiation 14. Structure and function of eukaryotic chromosomes. Springer, Berlin Heidelberg New York, pp 235–254

    Google Scholar 

  • Seabright, M (1971) A rapid banding technqiue for human chromosomes. Lancet ii:971–972

    Google Scholar 

  • Sola, L, Monaco, PJ, Rasch, EM (1990) Cytogenetics of bisexual/unisexual species of Poecilia. I. C-bands, AgNOR polymorphisms and sex chromosomes in three populations of Poecilia latipinna. Cytogenet Cell Genet 53:148–154

    Google Scholar 

  • Stock, AD, Mengden, GA (1975) Chromosome banding pattern conservation in birds and nonhomology of chromosome banding pattern between birds, turtles and amphibians. Chromosoma 50:69–77

    Google Scholar 

  • Sumner, AT (1972) A similar technique for demonstrating centromeric heterochromatin. Exp Cell Res 75:304–306

    Google Scholar 

  • Thiery, JP, Macaya, G, Bernardi, G (1976) An analysis of eukaryotic genomes by density gradient centrifugation. J Mol Biol 108:219–235

    Google Scholar 

  • Ueda, T, Sato, R, Kobayashi, J (1988) Silver-banded karyotypes of the rainbow trout and the brook trout and their hybrids. Jpn J Genet 63:219–226

    Google Scholar 

  • White, MJD (1978) Modes of speciation. W.H. Freeman, San Francisco

    Google Scholar 

  • Wiberg, UH (1983) Sex determination in the European eel (Anguilla anguilla, L.). Cytogenet Cell Genet 36:589–598

    Google Scholar 

  • Zenzes, MT, Voiculescu, I (1975) C-Banding patterns in Salmo trutta, a species of tetraploid origin. Genetica 45:531–536

    Google Scholar 

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by D. Schweizer

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Hellmer, A., Voiculescu, I. & Schempp, W. Replication banding studies in two cyprinid fishes. Chromosoma 100, 524–531 (1991). https://doi.org/10.1007/BF00352203

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