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Expression of a yeast gene can be blocked by insertion of short yeast DNA fragments between a UAS and the TATA box

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Abstract

We have constructed a plasmid, pOV10, which facilitates the introduction of putative upstream activating sequences (UAS) or upstream repressing sequences (URS) from yeast genes into plasmids containing CYC1-lacZ fusions. We have observed that the insertion of yeast sequences from 155 to 195 bp between the UAS and the TATA box of a CYC1-lacZ fusion gene can block β-galactosidase expression. It is suggested that this block is related to the formation of nucleosomes on the DNA.

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Authors and Affiliations

  1. Arturo Duperior, no. 4, Instituto de Investigaciones Biomédicas del C.S.I.C., E-28029, Madrid, Spain

    Olivier Vincent & Juana M. Gancedo

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  1. Olivier Vincent
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  2. Juana M. Gancedo
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Communicated by L. Frontali

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Vincent, O., Gancedo, J.M. Expression of a yeast gene can be blocked by insertion of short yeast DNA fragments between a UAS and the TATA box. Curr Genet 27, 387–389 (1995). https://doi.org/10.1007/BF00352109

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  • DOI: https://doi.org/10.1007/BF00352109

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