Abstract
A DNA insertion system, termed marked homologous recombination, was devised for the construction of complex yeast shuttle plasmids. This system, which is efficient, rapid and easy to use, should contribute to our understanding of gene-gene interactions in yeast cells.
Similar content being viewed by others
References
Botstein D, Falco C, Stewart S, Brennan M, Scherer S, Stinchcomb D, Struhl K, Davis R (1979) Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8:17–24
Daniel J (1993) Potentially rapid walking in cellular regulatory networks using the gene-gene interference method in yeast. Mol Gen Genet 240:245–257
Ito H, Fufuda Y, Murata K, Kimura A (1983) Transformation of intact yeast cells treated with alkali cations. J Bacteriol 153:163–168
Ma H, Kunes S, Schatz P, Botstein D (1987) Plasmid construction by homologous recombination in yeast. Gene 58:201–216
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual (2nd Edn.). Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Sherman F, Fink G, Hicks J (1987) Methods in yeast genetics: laboratory course manual. Cold Spring Harbor Laboratory Cold Spring Harbor, New York
Yanish-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103–119
Author information
Authors and Affiliations
Additional information
Communicated by K. Wolf
Rights and permissions
About this article
Cite this article
Daniel, J. DNA insertion system for complex yeast shuttle vectors. Curr Genet 27, 309–311 (1995). https://doi.org/10.1007/BF00352098
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00352098