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Promoter analysis of the bli-7/eas gene

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Abstract

Expression of the Neurospora crassa gene bli-7, (identical with eas, and ccg-2), is induced by blue light, as well as glucose- or nitrogen-starvation. A promoter analysis was performed by an assay that does not involve promoter-reporter constructs but rather the insertion of foreign DNA into the transcribed sequence. To detect regulatory elements a series of deletions in the upstream region was generated. The inducibility of the gene, in response to the three inducing conditions mentioned, is lost by eliminating the region between-1498 bp and-1017 bp upstream of the transcription start point. A segment with an apparently negative effect was found between-595 bp and-429 bp, as well as a stretch of DNA from-429 bp to-380 bp which may exert a positive influence after light induction.

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Authors and Affiliations

  1. Institut für Botanik, Universität Hannover, Herrenhäuser Strasse 2, D-30419, Hannover, Germany

    R. Kaldenhoff

  2. Max-Planck-Institut für molekulare Genetik, Ihnestrasse 73, D-14195, Berlin, Germany

    V. E. A. Russo

Authors
  1. R. Kaldenhoff
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  2. V. E. A. Russo
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Communicated by F.K. Zimmermann

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Kaldenhoff, R., Russo, V.E.A. Promoter analysis of the bli-7/eas gene. Curr Genet 24, 394–399 (1993). https://doi.org/10.1007/BF00351847

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  • DOI: https://doi.org/10.1007/BF00351847

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