Skip to main content
Log in

A gene involved in the biogenesis of cytochromes is co-transcribed with a ribosomal protein gene in wheat mitochondria

  • Original Articles
  • Published:
Current Genetics Aims and scope Submit manuscript

Abstract

Sequence analysis of a transcribed region of the wheat mitochondrial (mt) genome revealed two open reading frames (orfs) coding for proteins of 589 and 174 amino acids. Both genes are co-transcribed in a 2.6-kb RNA. The largest orf codes for a hydrophobic protein which bears similarity to a bacterial protein involved in the biogenesis of c-type cytochromes. Its corresponding RNA sequence is fully edited at 34 positions. The second orf encodes a protein homologous to the amino-terminal third of E. coli ribosomal protein S1, corresponding to the ribosome-binding domain of this protein. Its RNA sequence is edited at four positions, one of the edits creating a stop codon. The presence of both proteins in wheat mitochondria was demonstrated using specific antibodies raised against fusion proteins obtained in E. coli from the corresponding cDNAs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Anderson S, Bankier AT, Barrell BG, De Bruijn MHL, Coulson AR, Drouin J, Eperon IC, Nierlich DP, Roe BA, Sanger F, Schreir PH, Smith AJH, Staden R, Young IG (1981) Nature 290:457–465

    Google Scholar 

  • Beckman DL, Trawick DR, Kranz RG (1992) Genes Dev 6:268–283

    Google Scholar 

  • Binder S, Marchfelder A, Brennicke A, Wissinger B (1992) J Biol Chem 267:7615–7623

    Google Scholar 

  • Bland MM, Levings CS III, Matzinger DF (1986) Mol Gen Genet 204:8–16

    Google Scholar 

  • Blumenthal T, Carmichael GG (1979) Annu Rev Biochem 48:525–548

    Google Scholar 

  • Bonen L (1991) Curr Opin Genet Dev 1:515–522

    Google Scholar 

  • Bonnard G, Gualberto JM, Lamattina L, Grienenberger JM (1992) CRC Crit Rev Plant Sci 10:503–524

    Google Scholar 

  • Braun HP, Emmermann M, Kruft V, Schmitz UK (1992) Mol Gen Genet 231:217–225

    Google Scholar 

  • Chapdelaine Y, Bonen L (1991) Cell 65:465–472

    Google Scholar 

  • Covello PS, Gray MW (1991) Curr Genet 20:245–251

    Google Scholar 

  • Dewey RE, Levings CS III, Timothy DH (1986) Cell 44:439–449

    Google Scholar 

  • Falconet D, Lejeune B, Quétier F, Gray MW (1984) EMBO J 3:297–302

    Google Scholar 

  • Franzetti B, Zhou DX, Mache R (1992) Nucleic Acids Res 16:4153–4157

    Google Scholar 

  • Giorginis S, Subramanian AR (1980) J Mol Biol 141:393–408

    Google Scholar 

  • Gualberto JM, Wintz H, Weil JH, Grienenberger JM (1988) Mol Gen Genet 215:118–127

    Google Scholar 

  • Gualberto JM, Bonnard G, Lamattina L, Grienenberger JM (1991) Plant Cell 3:1109–1120

    Google Scholar 

  • Guerrier-Takada C, Subramanian AR, Cole PE (1983) J Biol Chem 258:13649–13652

    Google Scholar 

  • Hack E, Leaver CJ (1983) EMBO J 2:1783–1789

    Google Scholar 

  • Harlow E, Lane D (1988) Antibodies. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

    Google Scholar 

  • Hartl FU, Neupert W (1990) Science 247:930–938

    Google Scholar 

  • Hiesel R, Schobel W, Schuster W, Brennicke A (1987) EMBO J 6:29–34

    Google Scholar 

  • Hiratsuka J, Shimada H, Whittier R, Ishibashi T, Sakamoto M, Mori M, Kondo C, Honji Y, Sun CR, Meng BY, Li YQ, Kanno A, Nishikawa Y, Hirai A, Shinozaki K, Sugiura M (1989) Mol Gen Genet 217:185–194

    Google Scholar 

  • Hunt MD, Newton KJ (1991) EMBO J 10:1045–1052

    Google Scholar 

  • Laemmli UK (1970) Nature 227:680–685

    Google Scholar 

  • Leaver CJ, Harmey MA (1973) Biochem Soc Symp 38:175–193

    Google Scholar 

  • Newton KJ (1988) Annu Rev Plant Physiol Plant Mol Biol 39:503–532

    Google Scholar 

  • Nugent JM, Palmer JD (1991) Cell 66:473–481

    Google Scholar 

  • Oda K, Yamato K, Ohta E, Nakamura Y, Takemura M, Nozato N, Akashi K, Kanegae T, Ogura Y, Kohchi T, and Ohyama K (1992) J Mol Biol 223:1–7

    Google Scholar 

  • Ohyama K, Fukuzawa H, Kohchi T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H, Ozeki H (1986) Nature 322:572–574

    Google Scholar 

  • Pritchard AE, Seihlamer JJ, Mahalingam R, Sable CL, Venuti SE, Cummings DJ (1990) Nucleic Acids Res 18:173–180

    Google Scholar 

  • Quétier F, Lejeune B, Delorme S, Falconet D (1985) Molecular organization and expression of the mitochondrial genome of higher plants In: Douce R, Day DA (eds) Encyclopedia of Plant Physiology, Springer Verlag, Berlin, pp 25–35

    Google Scholar 

  • Ramseier TM, Winteler HV, Hennecke H (1991) J Biol Chem 266:7793–7803

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

    Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467

    Google Scholar 

  • Schnier J, Kimura M, Foulaki K, Subramanian AR, Isono K, Wittman-Liebold B (1982) Proc Natl Acad Sci USA 79:1008–1011

    Google Scholar 

  • Schuster W, Wissinger B, Unseld M, Brennicke A (1990) EMBO J 9:263–269

    Google Scholar 

  • Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimida H, Sugiura M (1986) EMBO J 5:2043–2049

    Google Scholar 

  • Smith DB, Johnson KS (1988) Gene 67:31–40

    Google Scholar 

  • Souza AP de, Jubier MF, Delcher E, Lancelin D, Lejeune B (1991) Plant Cell 3:1363–1378

    Google Scholar 

  • Stern DB, Newton KJ (1984) Plant Mol Biol Rep 2:8–15

    Google Scholar 

  • Subramanian AR, Rienhardt P, Kimura M, Suryanarayana T (1981) Eur J Biochem 119:245–249

    Google Scholar 

  • Takemura M, Oda K, Yamato K, Ohta E, Nakamura Y, Nozato N, Akashi K, Ohyama K (1992) Nucleic Acids Res 20:3199–3205

    Google Scholar 

  • Thomas JO, Szer W (1982) Prog Nucleic Acids Res Mol Biol 27:157–185

    Google Scholar 

  • Wahleithner JA, Wolstenholme DR (1988) Nucleic Acids Res 16:6897–6913

    Google Scholar 

  • Wissinger B, Schuster W, Brennicke A (1991) Cell 65:473–482

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by H. Kössel

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gonzalez, D.H., Bonnard, G. & Grienenberger, JM. A gene involved in the biogenesis of cytochromes is co-transcribed with a ribosomal protein gene in wheat mitochondria. Curr Genet 24, 248–255 (1993). https://doi.org/10.1007/BF00351799

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00351799

Key words

Navigation