Abstract
A cDNA encoding for the glucoamylase P enzyme (GAMP) of the fungus Hormoconis resinae was introduced into the cellulolytic filamentous fungus Trichoderma reesei under the control of the promoter of the major cellulase gene (cbh1) of Trichoderma. The transforming vector plasmid used was found to be integrated into the genome of T. reesei at various locations and in multiple copies. The size of the GAMP secreted by Trichoderma varied because of different glycosylation patterns. The best transformant strains secreted about 700 mg/l of active GAMP, which is 20-fold more than obtained with H. resinae.
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Aho S, Olkkonen V, Jalava T, Paloheimo M, Bühler R, Niku-Paavola M-L, Bamford DH, Korhola M (1991) Eur J Biochem 200: 643–649
Bailey MJ, Nevalainen KMH (1981) Enzyme Microbiol Technol 3: 153–157
Birnboim HC, Doly J (1979) Nucleic Acids Res 7: 1513–1523
Boyer HW, Roulland-Dussoix D (1969) J Mol Biol 41: 459–472
Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ (1979) Biochemistry 18: 5294–5299
Durand H, Baron M, Calmels T, Tiraby G (1988a) Classical and molecular genetics applied to Trichoderma reesei for the selection of improved cellulolytic industrial strains. In: Aubert J-P, Beguin P, Millet J (eds) Biochemistry and genetics of cellulose degradation. Academic Press, New York, pp 135–151
Durand H, Clanet M, Tiraby G (1988b) Enzyme Microbiol Technol 10: 341–345
Fagerström R, Vainio A, Suoranta K, Pakula T, Kalkkinen N, Torkkeli H (1990) J Gen Microbiol 136: 913–920
Garger SJ, Griffith DM, Grill LK (1983) Biochem Biophys Res 117: 835–842
Gritzali M, Brown RD Jr (1979) Adv Chem Ser 81: 237–260
Gruber F, Visser J, Kubicek CP, de Graaff LH (1990) Curr Genet 18: 71–76
Harkki A, Uusitalo J, Bailey M, Penttilä M, Knowles JKC (1989) Bio/Technology 7: 596–603
Henrissat B, Driguez H, Viet C, Schülein M (1985) Bio/Technology 3: 722–726
Hynes MJ, Corrick CM, King JA (1983) Mol Cell Biol 3: 1430–1439
Laemmli UK (1970) Nature 227: 680–685
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring, Harbor, New York
Manjunath P, Shenoy BC, Raghavendra Rao MR (1983) J Appl Biochem 5: 235–260
McCleary BV, Anderson MA (1980) Carbohydr Res 86: 77–96
Montenecourt BS (1983) Trends Biotechnol 1: 156–161
Montenecourt BS, Eveleigh DE (1979) Adv Chem Ser 181: 289–301
Nevalainen H, Penttilä M, Harkki A, Knowles JKC (1991) The molecular biology of Trichoderma and its application to the expression of both homologous and heterologous genes. In: Leong SA, Berka R (eds) Molecular industrial mycology. Marcel Dekker Inc., New York, pp 129–148
Nyyssönen E, Takkinen K, Alfthan K, Sizmann D, Saloheimo A, Siika-Aho M, Bailey M, Penttilä M, Keränen S (1992) 1st European Conference on Fungal Genetics, Nottingham, England, Abstract no P2/06
Pazur JH, Ando T (1959) J Biol Chem 234: 1966–1970
Penttilä M, Nevalainen H, Rättö M, Salminen E, Knowles J (1987) Gene 61: 155–164
Raeder U, Broda P (1985) Lett Appl Microbiol 1: 17–20
Reilly PJ (1979) Appl Biochem Bioeng 2: 185–206
Saha BC, Mitone T, Ueda W (1979) Stärke 31: 307–314
Saloheimo M, Barajas V, Niku-Paavola M-L, Knowles JKC (1989) Gene 85: 343–351
Saloheimo M, Niku-Paavola M-L (1991) Bio/Technology 9: 987–990
Shoemaker S, Schweickart V, Ladner M, Gelfand D, Kwock S, Myambo K, Innis M (1983) Bio/Technology 1: 691–696
Southern EM (1975) J Mol Biol 98: 503–517
Teeri T, Salovuori I, Knowles J (1983) Bio/Technology 1: 696–699
Towbin H, Staehelin T, Gordon J (1979) Proc Natl Acad Sci USA 76: 4350–4354
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Joutsjoki, V.V., Torkkeli, T.K. & Helena Nevalainen, K.M. Transformation of Trichoderma reesei with the Hormoconis resinae glucoamylase P (gamP) gene: production of a heterologous glucoamylase by Trichoderma reesei . Curr Genet 24, 223–228 (1993). https://doi.org/10.1007/BF00351796
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DOI: https://doi.org/10.1007/BF00351796